Anti cd16 pe

The dNK cells were cultured in 6-well plates (5 × 10⁵ cells/well) for 24 hours. The mononuclear cells were resuspended in staining buffer, and then immediately stained with a range of monoclonal antibodies, namely anti-CD56 TULY56 FITC (Catalog#: 11-0566-42, eBioscience, USA), anti-CD16 PE (Catalog#: 12-0168-42, eBioscience, USA), anti-CD39 APC (Catalog#: 17-0399-41, eBioscience, USA), anti-CD107 APC H4A3 (Catalog#: 560664, BD Pharmingen, USA), anti-CD314 PE (Catalog#: 557940, BD Pharmingen, USA), anti-CD336 PE (Catalog#: 558563, BD Pharmingen, USA), anti-CD337 PE (Catalog#: 558407, BD Pharmingen, USA), anti-CD73 PE (Catalog#: 550257, BD Pharmingen, USA), anti-Annexin V FITC (Catalog#: 556419, BD Pharmingen, USA), anti-DAPI (Catalog#: 564907, BD Pharmingen, USA) and anti-CD45 APCCY7 (Catalog#: 348805, BD Pharmingen, USA). After incubation at room temperature for 30 min, the cells were then washed and resuspended in PBS for flow cytometry analysis (CytoFLEX, eBioscience, USA). The strategy for multidimensional flow cytometry analysis is shown in the Supplementary Material, Figure S1.

Dextran sulfate sodium (DSS) and azoxymethane (AOM) were obtained from Yeasen Biotechnology Co., Ltd. (Shanghai, China). Antibodies of p-IκBα, p-p65, IκBα, p65, and GAPDH were purchased from Cell Signaling Technology (Danvers, MA, United States). Human TNF-α and the IL-6 ELISA kit were obtained from eBioscience (Vienna, Austria). Anti–CD16-PE, CD11c-FITC, CD11b-FITC, F4/80-PE, CD-3-APC, CD80-PE, and CD206-PE were obtained from eBioscience (Minneapolis, MN, United States). The Annexin V-FITC/PI apoptosis detection kit was obtained from BD Biosciences (San Jose, CA, United States). Calcein-AM/PI was acquired from Beyotime (Haimen, China). PDTC was purchased from MedchemExpress (Monmouth Junction, NJ, United States). Safflower polysaccharide (SPS) was obtained from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China).