Ebselen

HO-3867 (cat no: S7501), DFO (cat no: S6849), Dp44mT (cat no: S790), dexrazoxane HCl (cat no: S5651), salinomycin (cat no: S8129), ebselen (cat no: S6676), z.VAD.FMK (cat no: S7023), Nec-1 (cat no: S8037), Fer-1 (cat no: S7243), Lip-1 (cat no: S7699), and PFTα (cat no: S2929) were procured from Selleck Chemicals (USA). S-Benzylisothiourea (cat no: S9138) was ordered from Sigma-Aldrich (USA). The primary antibodies listed below were ordered from Cellular Signaling Technology (USA): caspase-3 (cat no:9662), Mcl-1 (cat no:94296), Bcl-2 (cat no:15071), Bcl-xL (cat no:2762), Bak (cat no:6947), Bax (cat no:94296), and DMT1 (cat no:15083). The following primary antibodies listed were procured from Abcam (USA): FPN1 (cat no: ab239583), TFR (cat no: ab214039), FTL (cat no: ab75973), FTH (cat no: ab183781), and p53 (cat no: ab26). The secondary antibody was procured from Abclonal (China). Sigma-Aldrich supplied all other routine chemicals.

Neurobasal A, Dulbecco’s Modified Eagle’s Medium (DMEM), FluoroBrite™ DMEM, supplement B27 (w/o antioxidants), fetal bovine serum (FBS), 0.25% trypsin/EDTA and penicillin/streptomycin were from Gibco (Life Technologies Ltd., Paisley, UK). The Cyto-toxicity Detection Kit and Cell Proliferation Reagent WST-1 were from Roche Diagnostics GmbH (Mannheim, Germany). Caspase-3 (Ac-DEVD-AMC) fluorogenic substrate and CM-H₂DCFDA were obtained from Enzo Life Sciences (New York, NY, USA) and Molecular Probes (Life Technologies Corporation, Eugene, OR, USA), respectively. Edaravone, ebselen and carnosic acid were purchased from Selleck Chemicals LLC (Houston, TX, USA). All other reagents were from Sigma (Sigma-Aldrich Chemie GmbH, Taufkirchen, Germany).