Recombinant TPNOX and other LbNOX variants were expressed in One Shot BL21(DE3) competent E. coli cells (Thermo Fisher Scientific) and purified as described previously26 (link). Proteins were immediately frozen in liquid nitrogen and stored at −80 °C until use. For all activity assays enzymes were cleaved with Enterokinase (EMD Millipore) to remove the N-terminal Hisx6 tag as described previously26 (link). In crystallization experiments non-cleaved proteins were used.
One shot bl21 de3 competent e coli cells
Manufactured by Thermo Fisher Scientific
One Shot BL21(DE3) competent E. coli cells are a laboratory reagent used for the transformation of expression plasmids into E. coli. These cells are designed to efficiently incorporate and express recombinant proteins under the control of the T7 promoter system.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using one shot bl21 de3 competent e coli cells
1
Recombinant Expression and Purification of LbNOX Variants
2
Recombinant Expression and Purification of LbNOX Variants
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