Frozen sections were stained with the Arcturus Histogene Kit. IFD regions and DP and DSC cells from anagen-phase follicles were dissected using pulled glass capillary tubes under magnification (Figure 1A ). RNA was extracted with the Arcturus PicoPure RNA Isolation Kit. cDNA was amplified from 500 pg of RNA per sample using the Nugen Ovation RNA-Seq System V2 and fragmented into 300bp segments using a Covaris sonicator. RNA-Seq libraries were constructed from 80 to 100 ng of cDNA with the Nugen Ovation Ultralow System V2. Sample concentration and quality was assessed with the Agilent Bioanalyzer.
Histogene kit
Manufactured by Arcturus
The Histogene Kit is a laboratory equipment designed for the analysis and detection of genetic materials. It provides a standardized platform for the preparation and processing of samples, enabling researchers to study the genetic composition and expression patterns within various biological samples.
Automatically generated - may contain errors
2 protocols using histogene kit
1
Transcriptome Analysis of Hair Follicles
2
Laser Capture Microdissection of Chicken Limb Bud
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