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Pe conjugated anti human oct4 oct3 antibody

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The PE-conjugated anti-human OCT4(OCT3) antibody is a tool for the detection and analysis of OCT4(OCT3), a key transcription factor involved in the regulation of embryonic stem cell pluripotency and self-renewal. This antibody is conjugated to the fluorescent dye Phycoerythrin (PE), allowing for sensitive and specific flow cytometric or fluorescence-based detection of OCT4(OCT3)-expressing cells.

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Lab products found in correlation

Facsaria 2, by BD (2 mentions) Percp cy5.5 conjugated mouse anti h2ax ps139, by BD (2 mentions) Apc conjugated anti gfpuv egfp antibody, by R&D Systems (2 mentions)

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Anti-Oct4 (3 citations)

2 protocols using pe conjugated anti human oct4 oct3 antibody

1

Monitoring Pluripotency and Genomic Integrity

Check if the same lab product or an alternative is used in the 5 most similar protocols
To directly monitor the eGFP expression levels, the wild type or mutant POU5F1-eGFP cells were dissociated with Accutase and subjected to FACS analysis with BD FACSAria II. To examine the levels of HLA-C protein, the cells were stained with PE-conjugated antibody specifically recognizing HLA-C (Millpore, Cat#MABF233). To carry out immunostaining of eGFP, POU5F1, or γH2AX, the cells were fixed with 2% PFA for 30 minutes, followed by overnight permeabilization in Methanol at −20°C. The treated cells were stained with the antibodies. PerCP-cy5.5-conjugated mouse anti-H2AX(pS139) was purchased BD Biosciences (Cat#564718); PE-conjugated anti-human OCT4(OCT3) antibody was from STEMCELL Technologies (Cat# 60093PE.1) and APC-conjugated anti-GFPuv/eGFP antibody is available from R&D Systems (Cat# IC4240A)
Diao Y., Fang R., Li B., Meng Z., Yu J., Qiu Y., Lin K.C., Huang H., Liu T., Marina R.J., Jung I., Shen Y., Guan K.L, & Ren B. (2017). A tiling1deletion based genetic screen for cis-regulatory element identification in mammalian cells. Nature methods, 14(6), 629-635.
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2

Monitoring Pluripotency and Genomic Integrity

Check if the same lab product or an alternative is used in the 5 most similar protocols
To directly monitor the eGFP expression levels, the wild type or mutant POU5F1-eGFP cells were dissociated with Accutase and subjected to FACS analysis with BD FACSAria II. To examine the levels of HLA-C protein, the cells were stained with PE-conjugated antibody specifically recognizing HLA-C (Millpore, Cat#MABF233). To carry out immunostaining of eGFP, POU5F1, or γH2AX, the cells were fixed with 2% PFA for 30 minutes, followed by overnight permeabilization in Methanol at −20°C. The treated cells were stained with the antibodies. PerCP-cy5.5-conjugated mouse anti-H2AX(pS139) was purchased BD Biosciences (Cat#564718); PE-conjugated anti-human OCT4(OCT3) antibody was from STEMCELL Technologies (Cat# 60093PE.1) and APC-conjugated anti-GFPuv/eGFP antibody is available from R&D Systems (Cat# IC4240A)
Diao Y., Fang R., Li B., Meng Z., Yu J., Qiu Y., Lin K.C., Huang H., Liu T., Marina R.J., Jung I., Shen Y., Guan K.L, & Ren B. (2017). A tiling1deletion based genetic screen for cis-regulatory element identification in mammalian cells. Nature methods, 14(6), 629-635.
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