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3 n morpholino propanesulfonic acid sodium salt

Manufactured by Merck Group
Sourced in France

3-(N-Morpholino)propanesulfonic acid sodium salt is a chemical compound commonly used as a buffer solution in various laboratory applications. It maintains a stable pH range, allowing for controlled environments in experiments and analysis. The product serves as a core functional component in laboratory equipment and procedures, but its specific intended use is not provided in this concise, unbiased description.

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2 protocols using 3 n morpholino propanesulfonic acid sodium salt

1

Functionalized Silica Nanoparticle Synthesis

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Sodium chloride (NormaPur), methylene blue (Alfa Aesar), potassium ferricyanide (III), and potassium hexacyanoferrate (II) (Sigma-Aldrich, St Quentin Fallavier, France) were used in the experiments without further purification. N-(3-Dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride (EDC); N-Hydroxy-sulfosuccinimide sodium salt (NHS); 3-(N-Morpholino)propanesulfonic acid sodium salt (MOPS) and tetraethoxyorthosilicate (TEOS); 3-(aminopropyl)triethoxysilane (APTS) were purchased from Sigma–Aldrich (St Quentin Fallavier, France). Citric acid was purchased from Merck (Nogent sur Marne, France). 2-[Methoxy(polyethyleneoxypropyl]-trimethoxysilane (PEOS), containing 3–6 ethylene oxide groups, was purchased from Gelest (Morrisville, PA, USA). From VWR (Strasbourg, France) 30% H2O2, HCl and NaOH solutions were obtained.
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2

Immobilization of Antibody on Gold Nanoparticles

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N-(3-dimethylaminopropyl)-N′-carbodiimide (EDC, 97%), N-hydroxysuccinimide
(NHS, 98%), 3-(N-morpholino)propanesulfonic acid
(MOPS, 99.5%), 3-(N-morpholino)propanesulfonic acid
sodium salt (99.5%), 2-(N-morpholino)ethanesulfonic
acid (MES, 99.5%), 2-(N-morpholino)ethanesulfonic
acid sodium salt (99%), hydroxylamine 50 weight % in H2O, isopropyl alcohol (IPA, 98%) were purchased from Sigma-Aldrich
(St. Louis, MO). Hydrochloric Acid, 10% v/v aqueous solution (HCl),
was purchased from Alfa Aesar (Ward Hill, MA). Gold nanospheres (40
nm, BioReady, carboxyl, 20 OD, 5 mL, water) were obtained from NanoComposix
(San Diego, CA). A rabbit polyclonal antibody against L. monocytogenes (PIPA130487) was purchased from
Fisher Scientific and used without further purification. All reagents
were used as received. Ultrapure type-1 water (18 MΩ cm) was
acquired from an Elga PURELAB purification system and was used for
all buffer preparations. Thermo Scientific Pierce 96-Well Plates,
Product No. 15041 was used for bicinchoninic acid (BCA) assay. Bovine
serum albumin (BSA) standard kit was purchased from Thermo Fisher
catalog #23225.
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