Fixed neurons were permeabilized by incubation with cold methanol at −20°C for 5 min and blocked with 2% BSA in PBS at RT for 1 hr. Neurons were immunostained against mouse-anti-V5 (1:2000 dilution, Invitrogen) and rabbit-anti-VP16 (1:2000 dilution, Abcam), followed by anti-mouse-alexafluoro488 (1:1000 dilution) and anti-rabbit-alexafluoro647 (1:1000 dilution) in 2% BSA solution in PBS. Neurons directly plated on the 48-well plate were imaged with 10x air objective in the Zeiss AxioObserver inverted confocal microscope and neurons plated on glass cover slips were imaged with 40x oil objective in the Zeiss AxioObserver inverted confocal microscope. Eight to ten fields of view were collected for each condition.
Rabbit anti vp16
Manufactured by Abcam
Rabbit-anti-VP16 is a polyclonal antibody raised in rabbits against the VP16 protein. VP16 is a viral transcriptional activator protein found in Herpes Simplex Virus 1. This antibody can be used for detection of VP16 in various applications.
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Lab products found in correlation
2 protocols using rabbit anti vp16
1
Immunostaining of Cultured Neurons
2
Immunostaining of Cultured Neurons
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Fixed neurons were permeabilized by incubation with cold methanol at −20°C for 5 min and blocked with 2% BSA in PBS at RT for 1 hr. Neurons were immunostained against mouse-anti-V5 (1:2000 dilution, Invitrogen) and rabbit-anti-VP16 (1:2000 dilution, Abcam), followed by anti-mouse-alexafluoro488 (1:1000 dilution) and anti-rabbit-alexafluoro647 (1:1000 dilution) in 2% BSA solution in PBS. Neurons directly plated on the 48-well plate were imaged with 10x air objective in the Zeiss AxioObserver inverted confocal microscope and neurons plated on glass cover slips were imaged with 40x oil objective in the Zeiss AxioObserver inverted confocal microscope. Eight to ten fields of view were collected for each condition.
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