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Pfn19a

Manufactured by Promega
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The PFN19A is a laboratory equipment product designed for specific scientific applications. It features a core function that enables researchers to perform critical tasks in their work. However, as a marketing specialist, I am not authorized to provide a detailed description of the product's intended use or make any claims about its capabilities. For more information, please consult the product's technical specifications or contact our sales team.

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Lab products found in correlation

Pfn21a vector, by Promega (2 mentions) Tnt sp6 high yield wheat germ reaction, by Promega (2 mentions) Tnt quick coupled transcription translation system, by Promega (1 mentions)

Spelling variants of this product

PFN19A vector (2 citations)

3 protocols using pfn19a

1

Synthesis and RNA Pulldown of IGF2BP1

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IGF2BP1 cDNA (NM_006546.3) was purchased from Gegecopoea. IGF2BP1 coding region was amplified by PCR and cloned into pFN19A (HaloTag®7) T7 SP6 Flexi vector (Promega). IGF2BP1 deletion constructs were generated by inverse PCR using primers described in Supplementary Table 3. All clones were verified by DNA sequencing. The HaloTag fusion proteins were synthesized by incubating 3 μg plasmid with in vitro TNT® Quick-coupled Transcription/Translation System (Promega). Synthesized proteins were subjected to RNA Pulldown Assay.
Hosono Y., Niknafs Y.S., Prensner J.R., Iyer M.K., Dhanasekaran S.M., Mehra R., Pitchiaya S., Tien J., Escara-Wilke J., Poliakov A., Chu S.C., Saleh S., Sankar K., Su F., Guo S., Qiao Y., Freier S.M., Bui H.H., Cao X., Malik R., Johnson T.M., Beer D.G., Feng F.Y., Zhou W, & Chinnaiyan A.M. (2017). Oncogenic Role of THOR, a Conserved Cancer/Testis Long Noncoding RNA. Cell, 171(7), 1559-1572.e20.
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2

Expression and Analysis of Chromatin Regulators

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EZH2, BMI1, RING1B, EED and sub-domains were cloned into pFN19A and pFN21A vectors (Promega) in accordance to the manufacturer’s instructions. Fusion proteins were expressed in TNT® SP6 High-Yield Wheat Germ Reaction (Promega) following manufacturer’s instruction, or transfected into HEK293 cells for transient expression. A total of 2.0μl of cell-free reaction containing the HaloTag® fusion protein or 10ug total proteins from HEK293 cell lysates were resolved on SDS gels and blotted using anti-Halo or indicated antibodies (Supplementary Table 3) Dilutions are listed in Supplementary Table 3.
Cao Q., Wang X., Zhao M., Yang R., Malik R., Qiao Y., Poliakov A., Yocum A.K., Li Y., Chen W., Cao X., Jiang X., Dahiya A., Harris C., Feng F.Y., Kalantry S., Qin Z.S., Dhanasekaran S.M, & Chinnaiyan A.M. (2014). The Central Role of EED in the Orchestration of Polycomb Group Complexes. Nature communications, 5, 3127.
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3

Expression and Analysis of Chromatin Regulators

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EZH2, BMI1, RING1B, EED and sub-domains were cloned into pFN19A and pFN21A vectors (Promega) in accordance to the manufacturer’s instructions. Fusion proteins were expressed in TNT® SP6 High-Yield Wheat Germ Reaction (Promega) following manufacturer’s instruction, or transfected into HEK293 cells for transient expression. A total of 2.0μl of cell-free reaction containing the HaloTag® fusion protein or 10ug total proteins from HEK293 cell lysates were resolved on SDS gels and blotted using anti-Halo or indicated antibodies (Supplementary Table 3) Dilutions are listed in Supplementary Table 3.
Cao Q., Wang X., Zhao M., Yang R., Malik R., Qiao Y., Poliakov A., Yocum A.K., Li Y., Chen W., Cao X., Jiang X., Dahiya A., Harris C., Feng F.Y., Kalantry S., Qin Z.S., Dhanasekaran S.M, & Chinnaiyan A.M. (2014). The Central Role of EED in the Orchestration of Polycomb Group Complexes. Nature communications, 5, 3127.
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