Streptomycin r5

Manufactured by Wisent

Sourced in Canada

Streptomycin (R5) is a laboratory equipment product used for the detection and analysis of streptomycin, an antibiotic compound. It provides a reliable and efficient method for quantifying the presence and concentration of streptomycin in various samples.

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Lab products found in correlation

Live dead fixable dead cell stain, by Thermo Fisher Scientific (2 mentions) Cd16 apc cy7 3g8, by BD (2 mentions) Cd57 pe tb01, by Thermo Fisher Scientific (2 mentions) Nkg2c pe vio770 rea205, by Miltenyi Biotec (2 mentions) Nkg2a apc rea110, by Miltenyi Biotec (2 mentions)

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Streptomycin (137 citations)

2 protocols using streptomycin r5

Multicolor Flow Cytometry Analysis of Immune Cells

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Frozen PBMCs were thawed and resuspended in RPMI 1640; 5% fetal bovine serum; 2 mM L-glutamine; 50 international units/mL penicillin; 50 mg/mL streptomycin (R5) (all from Wisent, Inc., Saint-Jean-Baptiste, QC, Canada). LIVE/DEAD fixable dead cell stain (Invitrogen, Saint Laurent, QC, Canada) was added to the PBMCs as per manufacturer’s directions before surface staining cells using a panel that included the following fluorochrome conjugated antibodies to CD3-BV785 (clone OKT3), CD14-BV785 (M5E2), CD19-BV785 (HIB19), CD56-BV605 (HCD56) (all from BioLegend, San Diego, CA), CD57-PE (TB01) (Life Technologies, Burlington, ON, Canada), CD16-APC-Cy7 (3G8) (BD Bioscience, Baltimore, MD), NKG2C-PE-Vio770 (REA205) and NKG2A-APC (REA110) (Miltenyi Biotec, Auburn, CA).

Alsulami K., Sadouni M., Tremblay-Sher D., Baril J.G., Trottier B., Dupuy F.P., Chartrand-Lefebvre C., Tremblay C., Durand M, & Bernard N.F. (2022). High frequencies of adaptive NK cells are associated with absence of coronary plaque in cytomegalovirus infected people living with HIV. Medicine, 101(38), e30794.

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Phenotyping of Human Immune Cells

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Frozen PBMCs were thawed and resuspended in RPMI 1640; 5% fetal bovine serum (FBS); 2 mM L-glutamine; 50 international units/mL penicillin; 50 µg/mL streptomycin (R5) (all from Wisent, Inc., Saint Jean-Baptiste, QC, Canada). LIVE/DEAD fixable dead cell stain (Invitrogen, Saint Laurent, QC, Canada) was added to the PBMCs as per the manufacturer’s directions before the cells were surface stained using a panel that included the following fluorochrome conjugated antibodies to CD3-BV785 (clone OKT3), CD14-BV785 (M5E2), CD19-BV785 (HIB19), CD56-BV605 (HCD56) (all from BioLegend, San Diego, CA, USA), CD57-PE (TB01) (Life Technologies, Burlington, ON, Canada), CD16-APC-Cy7 (3G8) (BD Bioscience, Baltimore, MD, USA), NKG2C-PE-Vio770 (REA205) and NKG2A-APC (REA110) (Miltenyi Biotec, Auburn, CA, USA) for 20 min at 4 °C.

Alsulami K., Dupuy F.P., Gilbert L., Messier-Peet M., Durand M., Tremblay C., Routy J.P., Bruneau J., Baril J.G., Trottier B, & Bernard N.F. (2023). The Frequency and Function of NKG2C+CD57+ Adaptive NK Cells in Cytomagalovirus Co-Infected People Living with HIV Decline with Duration of Antiretroviral Therapy. Viruses, 15(2), 323.

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