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Cd43 s7

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CD43 (S7) is a cell surface glycoprotein expressed on the majority of hematopoietic cells, including T cells, B cells, monocytes, and granulocytes. It functions as a sialoglycoprotein involved in cell-cell and cell-matrix interactions.

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Lab products found in correlation

B220 ra3 6b2, by BioLegend (1 mentions) Cd93 aa4, by BioLegend (1 mentions) Cd24 m1 69, by BioLegend (1 mentions) Cd11b m1 70, by Thermo Fisher Scientific (1 mentions) Cd80 16 10a1, by BioLegend (1 mentions) Cd86 gl 1, by BioLegend (1 mentions) Cd69 h1.2f3, by BioLegend (1 mentions) Cd44 im7, by BioLegend (1 mentions) Facscanto 10c, by BD (1 mentions) Cd3e 145 2c11, by Thermo Fisher Scientific (1 mentions) Cd4 gk1, by Thermo Fisher Scientific (1 mentions) Cd23 b3b4, by BioLegend (1 mentions) Cd5 53 7, by BioLegend (1 mentions) Cd9 mz3, by BioLegend (1 mentions) Cd21 cd35 7e9, by BioLegend (1 mentions) Anti rabbit igg, by BioLegend (1 mentions) Cd45.1 a20, by BioLegend (1 mentions) Cd45.2 104, by BioLegend (1 mentions) Cd8 53 6, by BioLegend (1 mentions) Igd 11 26c 2a, by Thermo Fisher Scientific (1 mentions)

2 protocols using cd43 s7

1

Multiparametric Flow Cytometry Analysis

Cited 1 time
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Cell suspensions from mouse liver, BM (one femur and tibia), spleen, thymus, mesenteric lymph nodes, and peritoneal cavity were processed as previously described (47 (link)), and data were acquired on a FACSCanto10c (BD) and analyzed with FlowJo Software (Tree Star).
The following anti-mouse antibodies were used: B220 (RA3-6B2, BioLegend), BAFFR (7H22-E16,BD), BP-1 (6C3, BioLegend), CD3e (145-2C11, eBioscience), CD4 (GK1.5, eBioscience), CD5 (53-7.3, BioLegend), CD8 (53-6.7, BioLegend), CD9 (MZ3, BioLegend), CD11b (M1/70, eBioscience), CD19 (6D5, 1D3, BioLegend), CD21/CD35 (7E9, BioLegend), CD23 (B3B4, BioLegend), CD24 (M1/69, BioLegend), CD25 (PC61, Biolgend), CD43 (S7, BioLegend), CD44 (IM7, BioLegend), CD45.1 (A20, BioLegend), CD45.2 (104, BioLegend), CD69 (H1.2F3, BioLegend), CD80 (16-10A1, BioLegend), CD86 (GL1, BioLegend), CD93 (AA4.1, BioLegend), DAPI (BIOTIUM), Gr-1 (RB6-8C5, eBioscience), IgDa (AMS-9.1, BioLegend), IgD (11-26c, BioLegend), IgMa (DS-1, MA-69, BioLegend), IgM (Il/41, RMM-1, BioLegend), TCRγδ (GL3, BioLegend), Live dead dye (Zombie Aqua Dye, BioLegend), Live dead dye (Zombie NIR, BioLegend), pERK (4B11B69, BD), pPLCγ2 (K86-1161, BD), Lin28b (AP1485C, ABGENT), and anti-rabbit IgG (BioLegend).
Xu X., Deobagkar-Lele M., Bull K.R., Crockford T.L., Mead A.J., Cribbs A.P., Sims D., Anzilotti C, & Cornall R.J. (2020). An ontogenetic switch drives the positive and negative selection of B cells. Proceedings of the National Academy of Sciences of the United States of America, 117(7), 3718-3727.
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2

Multiparameter Flow Cytometry for Analyzing Lymphocyte Populations

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For analysis of lymphocyte populations, single-cell suspensions were prepared from homogenised spleens or bone marrow. For splenic suspension preparation, erythrocytes were destroyed with lysis buffer (BD Biosciences). The cells were treated with the appropriate combination of the following antibodies: CD16/32 (Fc block) (93) (eBioscience), B220 (RA3-6B2) (eBioscience), CD19 (eBio-1D3) (eBioscience), IgD (11-26c.2a) (eBioscience), IgM (II/41) (BioLegend), CD43 (S7) (BioLegend), CD24 (M1/69) (BD Biosciences), CD21 (7G6) (BD Biosciences), and BP-1/Ly-51 (6C3) (BioLegend). To identify splenic plasma cells or plasma cells from lymph node in vivo, cells positive for CD138 (281.2) (eBioscience) and negative for IgD (11-26c.2a) were considered plasma cells. For analysis of in vitro B-cell cultures, after blocking Fc receptors using anti-CD16/32 antibody, CTV-labelled cells were stained with the antibodies CD138 (281.2) and IgG1 (A85.1). For detection of vimentin using flow cytometry, anti-vimentin antibody (ERP3776) (Abcam) was used.
Tsui C., Maldonado P., Montaner B., Borroto A., Alarcon B., Bruckbauer A., Martinez-Martin N, & Batista F.D. (2018). Dynamic reorganisation of intermediate filaments coordinates early B-cell activation. Life Science Alliance, 1(5), e201800060.
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