Pc7 cd41

Manufactured by Beckman Coulter

The PC7-CD41 is a reagent used in flow cytometry analysis. It is designed to detect and quantify CD41 antigen expression on the surface of platelets. The PC7-CD41 reagent contains a fluorescent dye that binds to the CD41 antigen, enabling the identification and enumeration of platelet populations in a sample.

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Lab products found in correlation

Cd42b pe, by Beckman Coulter (2 mentions) Navios 10 3, by Beckman Coulter (2 mentions) Alexafluor750 cd235a, by Beckman Coulter (2 mentions) Cd56 pe, by Beckman Coulter (1 mentions) Cd19 fitc, by Beckman Coulter (1 mentions) Fitc cd38, by Beckman Coulter (1 mentions) Cd4 apc, by Beckman Coulter (1 mentions) Kaluza 1 3 1, by Beckman Coulter (1 mentions) Cytoflex b5 r3 v5, by Beckman Coulter (1 mentions) Pc7 cd34, by Beckman Coulter (1 mentions) Kaluza 1, by Beckman Coulter (1 mentions)

2 protocols using pc7 cd41

Immunophenotypic Characterization of Hematopoietic Cells

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Transduced HSPCs were sorted based on expression of GFP or Cerulean. In vitro differentiation was analyzed on day 14 of culture. Flow cytometry analyses was performed on a Navios 10/3 or a CytoFLEX B5-R3-V5 (both Beckman Coulter). Kaluza 1.3/1.5 (Beckman Coulter) or FlowJo V10 were used for data analysis. Staining and measurement were performed according to standard protocols as previously described for human cells^{44 (link)}, using the antibodies FITC-CD8 (B9.11), FITC-CD19 (89B), FITC-CD38 (T16), FITC-CD41 (P2), FITC-CD66b (80H3), PE-CD42b (SZ2), PE-CD56 (IM2073U), PE-CD123 (9F5), PE-CD117 (95C3), PC5.5-CD14 (RMO52), PC7-CD3 (UCHT1), PC7-CD34 (581), PC7-CD41 (P2), PC7-CD235a (11E4B-7-6), APC-CD4 (13B8.2), APC-CD13 (Immu103.44), APC-CD34 (581), APC-CD45RA (2H4LDH11LDB9), AlexaFluor750-CD19 (89B), AlexaFluor750-CD235a (11E4B-7-6), KromOrange-CD3 (UCHT1) (all Beckman Coulter), PE-CD36 (CB38), PC7-CD66b (G10F5), and APC-CD42b (HIP1) (Becton Dickinson).

Schwarzer A., Emmrich S., Schmidt F., Beck D., Ng M., Reimer C., Adams F.F., Grasedieck S., Witte D., Käbler S., Wong J.W., Shah A., Huang Y., Jammal R., Maroz A., Jongen-Lavrencic M., Schambach A., Kuchenbauer F., Pimanda J.E., Reinhardt D., Heckl D, & Klusmann J.H. (2017). The non-coding RNA landscape of human hematopoiesis and leukemia. Nature Communications, 8, 218.

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HSPC Flow Cytometry Immunophenotyping

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Transduced HSPCs were sorted based on GFP-expression. Flow Cytometry was performed on a Navios 10/3 (Beckman Coulter). Kaluza 1.2 (Beckman Coulter) was used for data analysis. Staining and measuring were performed according to standard protocols and as described previously using the antibodies PE-CD42b, PC5.5-CD13, PC7-CD41, PC7-CD117, AlexaFluor®750-CD235a (all Beckman Coulter), APC-CD36, APC-CD42b (both Becton Dickinson) and PacificBlue-CD71 (Exbio) [14 (link)].

Emmrich S., Streltsov A., Schmidt F., Thangapandi V.R., Reinhardt D, & Klusmann J.H. (2014). LincRNAs MONC and MIR100HG act as oncogenes in acute megakaryoblastic leukemia. Molecular Cancer, 13, 171.

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