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E6 sc 460

Manufactured by Santa Cruz Biotechnology
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The E6 (sc-460) is a laboratory instrument produced by Santa Cruz Biotechnology. The core function of this product is to perform immunoprecipitation assays, a widely used technique in molecular biology research.

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Lab products found in correlation

Polyvinylidene difluoride membrane, by Merck Group (2 mentions) Protein loading buffer, by Thermo Fisher Scientific (2 mentions) Sc 7351, by Santa Cruz Biotechnology (2 mentions) Semi dry electroblotting, by Bio-Rad (2 mentions) Sc 1050, by Santa Cruz Biotechnology (2 mentions) Sc 789, by Santa Cruz Biotechnology (2 mentions) Bca protein assay, by Merck Group (2 mentions) Sc30435, by Santa Cruz Biotechnology (2 mentions) Sc 126, by Santa Cruz Biotechnology (2 mentions) Sc 200, by Santa Cruz Biotechnology (2 mentions) Sc 188 c 19, by Santa Cruz Biotechnology (2 mentions) Sc 520, by Santa Cruz Biotechnology (2 mentions) Sc 32233, by Santa Cruz Biotechnology (2 mentions)

Close spelling variants of this product

Sc-460 (4 citations)

2 protocols using e6 sc 460

1

Western Blot Protein Analysis Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Using BCA protein assay (Sigma Aldrich), equal amounts of proteins were denatured with 1X protein loading buffer (ThermoFisher Scientific) at 100°C for 10 minutes. Samples were electrophoresed in an SDS-PAGE and transferred onto a polyvinylidene difluoride membrane (Millipore) by semidry electroblotting (Bio-Rad) as described.22 (link) Membranes were probed with primary antibodies for HRas (1:200; sc-520, Santa Cruz Biotechnology), c-Myc (1:200; sc-789, Santa Cruz Biotechnology), p53 (1:500; sc-126, Santa Cruz Biotechnology), COX2 (1:1000; clone CX229, Caymann Chemicals), E6 (sc-460, Santa Cruz Biotechnology), E1A (1:1000, clone M73, Millipore), ATF4 (1:250, sc-200, Santa Cruz Biotechnology), CHOP (1:1000, sc-7351, Santa Cruz Biotechnology), SCD1 (1:300, sc30435, goat polyclonal, Santa Cruz), β-actin (1:1000, A1978, Sigma-Aldrich), BiP (1:250, sc-1050, Santa Cruz Biotechnology), HA (1:1000, H6908 Sigma), SCD-5 (AP53809PU-N, 2BScientific Ltd), ATF-3 (sc-188(C-19), Santa Cruz), and against GAPDH (1:1500, sc-32233, Santa Cruz). The antibody against Bap31 was kindly provided by Dr Gordon Shore. Antibody binding was detected by enhanced chemiluminescence (Thermo Sientific) and visualised on film.
AbuAli G., Chaisaklert W., Stelloo E., Pazarentzos E., Hwang M.S., Qize D., Harding S., Al-Rubaish A., Alzahrani A.H., Al-Ali A., Sanders T., Aboagye E.O, & Grimm S. (2014). The anticancer gene ORCTL3 targets stearoyl-CoA desaturase-1 for tumour-specific apoptosis. Oncogene, 34(13), 1718-1728.
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2

Western Blot Protein Analysis Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Using BCA protein assay (Sigma Aldrich), equal amounts of proteins were denatured with 1X protein loading buffer (ThermoFisher Scientific) at 100°C for 10 minutes. Samples were electrophoresed in an SDS-PAGE and transferred onto a polyvinylidene difluoride membrane (Millipore) by semidry electroblotting (Bio-Rad) as described.22 (link) Membranes were probed with primary antibodies for HRas (1:200; sc-520, Santa Cruz Biotechnology), c-Myc (1:200; sc-789, Santa Cruz Biotechnology), p53 (1:500; sc-126, Santa Cruz Biotechnology), COX2 (1:1000; clone CX229, Caymann Chemicals), E6 (sc-460, Santa Cruz Biotechnology), E1A (1:1000, clone M73, Millipore), ATF4 (1:250, sc-200, Santa Cruz Biotechnology), CHOP (1:1000, sc-7351, Santa Cruz Biotechnology), SCD1 (1:300, sc30435, goat polyclonal, Santa Cruz), β-actin (1:1000, A1978, Sigma-Aldrich), BiP (1:250, sc-1050, Santa Cruz Biotechnology), HA (1:1000, H6908 Sigma), SCD-5 (AP53809PU-N, 2BScientific Ltd), ATF-3 (sc-188(C-19), Santa Cruz), and against GAPDH (1:1500, sc-32233, Santa Cruz). The antibody against Bap31 was kindly provided by Dr Gordon Shore. Antibody binding was detected by enhanced chemiluminescence (Thermo Sientific) and visualised on film.
AbuAli G., Chaisaklert W., Stelloo E., Pazarentzos E., Hwang M.S., Qize D., Harding S., Al-Rubaish A., Alzahrani A.H., Al-Ali A., Sanders T., Aboagye E.O, & Grimm S. (2014). The anticancer gene ORCTL3 targets stearoyl-CoA desaturase-1 for tumour-specific apoptosis. Oncogene, 34(13), 1718-1728.
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