Vγ4 uc3 10a6

Manufactured by BioLegend

The Vγ4 (UC3-10A6) is a monoclonal antibody that recognizes the Vγ4 chain of the T cell receptor (TCR) gamma chain. It is commonly used in flow cytometry and other immunoassays to identify and study Vγ4+ T cells.

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2 protocols using vγ4 uc3 10a6

Multiparameter Flow Cytometry Analysis

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Anti-mouse CD45 (30-F11) and IL-17A (TC11-18H10) antibodies were obtained from BD Biosciences (Franklin Lakes, NJ). Anti-mouse CD11b (M1/70), CD11c (N418), TCRγδ (eBioGL3), and Ki67 (SolA15) antibodies were purchased from eBioscience (San Diego, CA). Anti-mouse F4/80 (BM8), Ly-6G (1A8), MHC class II (M5/114.15.2), TCRβ (H57-597), and Vγ4 (UC3-10A6) antibodies were purchased from BioLegend (San Diego, CA). For intracellular staining, cells were stimulated for 3 h with 50 ng/ml PMA (phorbol myristate acetate; Sigma-Aldrich, St Louis, MO) and 1 μg/ml ionomycin (Wako, Osaka, Japan) in the presence of 10 μg/ml brefeldin A (Sigma-Aldrich) or put 10 μg/ml brefeldin A in the collagenase solution, and then fixed and permeabilized with Cytofix/Cytoperm buffer (BD Biosciences). Flow cytometry was performed using LSRFortessa (BD Biosciences) and analyzed with FlowJo (TreeStar, San Carlos, CA).

Nakamizo S., Honda T., Adachi A., Nagatake T., Kunisawa J., Kitoh A., Otsuka A., Dainichi T., Nomura T., Ginhoux F., Ikuta K., Egawa G, & Kabashima K. (2017). High fat diet exacerbates murine psoriatic dermatitis by increasing the number of IL-17-producing γδ T cells. Scientific Reports, 7, 14076.

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Flow Cytometric Analysis of Skin-Infiltrating T Cells

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Fluorochrome-labeled mAbs including anti-mouse CD3 (17A2, RRID: AB_1595492), γδTCR (GL3, RRID: AB_1595492), Vγ4 (UC3-10A6, RRID: AB_10569353), Vδ4 (GL2, RRID: AB_1877234), CD45 (30-F11, RRID: AB_312973), CD11b (M1/70, RRID: AB_312791), Gr-1 (RB6-8C5, RRID: AB_313377), and IL-17A (TC11-18H10.1, RRID: AB_2125010) and IL1R1 (DIH9, RRID: AB_2687367) were purchased from Biolegend. Anti-human γδTCR (REA591, Cat # 130-113-508) was purchased from Miltenyi Biotec and anti-human CD3 (UCHT1, Cat # 550795) was purchased from BD bioscience. For intracellular IL-17 staining, mouse skin cells or LN cells were treated with GolgiPlug (Biolegend) at 37°C for 4 h. Cells were first blocked with anti-CD16/32 and then stained with different cell surface Abs at 4°C for 20 min. Cells were then fixed, permeabilized and stained intracellularly at 4°C overnight for IL-17A. The relevant isotype control mAbs were also used. Samples were harvested with BD FACS Canto and analyzed with FlowJo software (TreeStar).

Liu N., Qin H., Cai Y., Li X., Wang L., Xu Q., Xue F., Chen L., Ding C., Hu X., Tieri D., Rouchka E.C., Yan J, & Zheng J. (2022). Dynamic trafficking patterns of IL-17-producing γδ T cells are linked to the recurrence of skin inflammation in psoriasis-like dermatitis. eBioMedicine, 82, 104136.

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