Log In Sign up
The largest database of trusted experimental protocols

Freezeview

Manufactured by Harvard Apparatus
Visit Supplier

FreezeView is a laboratory instrument designed for controlled freezing and imaging of samples. It allows for real-time observation of frozen samples during the freezing process.

Automatically generated - may contain errors

Lab products found in correlation

Stainless steel grid shock floor, by Harvard Apparatus (3 mentions) Freezeframe, by Harvard Apparatus (3 mentions)

Close spelling variants of this product

FreezeView Software FreezeView automated analysis

3 protocols using freezeview

1

Fear Conditioning in Rats: Tone Pre-exposure

Check if the same lab product or an alternative is used in the 5 most similar protocols
Female MAM- and saline-treated rats were placed into a 30.5 × 25.4 × 30.5 cm3 square conditioning chamber with metal walls and a stainless steel grid shock floor (Coulbourn Instruments, Whitehall, PA, USA). Rats were randomly assigned into two groups: tone pre-exposed and non-exposed. Pre-exposed rats were presented with a 20 second tone over 16 trials with a pseudo-random ITI averaging 2 minutes. Immediately following tone exposure, rats underwent an established fear conditioning procedure in which a 20 second tone co-terminated with a mild (0.8 mA, 0.5 sec) foot shock. The tone-shock pairing was presented 4 times with a pseudo-random ITI averaging 2 minutes. Twenty-four hours after conditioning, rats were returned to the conditioning chamber and re-exposed to the 20 second tone over 4 trials. Behavior was video recorded at each stage and freezing behavior was analyzed off-line using FreezeFrame and FreezeView software (Coulbourn Instruments). Freezing was defined as behavior below a motion index threshold of 10 lasting at least 1 second.
Perez S.M., Donegan J.J, & Lodge D.J. (2019). Effect of estrous cycle on schizophrenia-like behaviors in MAM exposed rats. Behavioural brain research, 362, 258-265.
+ Open protocol
+ Expand
2

Latent Inhibition Test for Schizophrenia

Check if the same lab product or an alternative is used in the 5 most similar protocols
The latent inhibition test was used to model a positive symptom of schizophrenia. Briefly, rats were placed into a 30.5×25.4×30.5 cm3 square conditioning chamber with metal walls and a stainless steel grid shock floor (Coulbourn Instruments) and randomly assigned to two groups: tone pre-exposed and non-exposed. Pre-exposed rats were presented with a 20 sec tone over 16 trials with a pseudorandom intertrial interval averaging 2 min. Immediately following tone exposure, rats underwent an established fear conditioning procedure in which the 20 sec tone co-terminated with a mild (0.8 mA, 0.5 sec) footshock. The tone-shock pairing was presented 4 times with a pseudorandom intertrial interval averaging 2 min. Twenty-four hours after conditioning, rats were returned to the conditioning chamber and re-exposed to the 20 sec tone over 4 trials. Behavior was video-recorded at each stage and freezing behavior was analyzed off-line using FreezeFrame and FreezeView software (Coulbourn Instruments). Freezing was defined as behavior below a motion index threshold of 10 lasting at least 1 sec. The percentage of latent inhibition was determined using the following formula: % LI = 100 − NoTone/Tone x 100.
Donegan J.J., Tyson J.A., Branch S.Y., Beckstead M.J., Anderson S.A, & Lodge D.J. (2016). Stem cell derived interneuron transplants as a treatment for schizophrenia: preclinical validation in a rodent model. Molecular psychiatry, 22(10), 1492-1501.
+ Open protocol
+ Expand
3

Latent Inhibition Test for Schizophrenia

Check if the same lab product or an alternative is used in the 5 most similar protocols
The latent inhibition test was used to model a positive symptom of schizophrenia. Briefly, rats were placed into a 30.5×25.4×30.5 cm3 square conditioning chamber with metal walls and a stainless steel grid shock floor (Coulbourn Instruments) and randomly assigned to two groups: tone pre-exposed and non-exposed. Pre-exposed rats were presented with a 20 sec tone over 16 trials with a pseudorandom intertrial interval averaging 2 min. Immediately following tone exposure, rats underwent an established fear conditioning procedure in which the 20 sec tone co-terminated with a mild (0.8 mA, 0.5 sec) footshock. The tone-shock pairing was presented 4 times with a pseudorandom intertrial interval averaging 2 min. Twenty-four hours after conditioning, rats were returned to the conditioning chamber and re-exposed to the 20 sec tone over 4 trials. Behavior was video-recorded at each stage and freezing behavior was analyzed off-line using FreezeFrame and FreezeView software (Coulbourn Instruments). Freezing was defined as behavior below a motion index threshold of 10 lasting at least 1 sec. The percentage of latent inhibition was determined using the following formula: % LI = 100 − NoTone/Tone x 100.
Donegan J.J., Tyson J.A., Branch S.Y., Beckstead M.J., Anderson S.A, & Lodge D.J. (2016). Stem cell derived interneuron transplants as a treatment for schizophrenia: preclinical validation in a rodent model. Molecular psychiatry, 22(10), 1492-1501.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!