The largest database of trusted experimental protocols

Mouse anti ssea4

Manufactured by Thermo Fisher Scientific

The Mouse anti-SSEA4 is a primary antibody that recognizes the stage-specific embryonic antigen 4 (SSEA4) marker. SSEA4 is a glycolipid surface antigen expressed on human embryonic stem cells and other pluripotent stem cells. This antibody can be used to identify and characterize these cell types in research applications.

Automatically generated - may contain errors

2 protocols using mouse anti ssea4

1

Immunofluorescence Staining Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following antibodies were used: rabbit anti-GFAP (DAKO Agilent, Santa Clara, CA, Z0334), rabbit anti-caspase-6 (Cell Signaling Technologies, Danvers, MA, 9762), rabbit anti-Caspase-6 (abcam, Cambridge, UK, ab185645), rabbit anti-D225 (Chen et al., 2013 (link)), mouse anti-GFAP (Sigma, GA5), mouse anti-pSer13-GFAP (KT13 [Sekimata et al., 1996 (link)]), mouse anti-pan Actin, mouse anti-Tra-1–60, mouse anti-SSEA4, rabbit anti-Oct4, rabbit anti-Sox2, and Alexa 488- and Alexa 594-congujated goat anti mouse or rabbit antibodies (Thermo Fisher Scientific, Waltham, MA).
+ Open protocol
+ Expand
2

Generation of hiPSCs-PB-TO-hNGN2 Stable Cell Line

Check if the same lab product or an alternative is used in the 5 most similar protocols
An hiPSCs-PB-TO-hNGN2 stable cell line was generated as previously described.70 (link) Briefly, KOLF2.1J wildtype cells were transfected with piggyBac plasmid carrying rrTA and Ngn2-Puro cassette (plasmids gifted from Michael Ward). Transfected cells were selected for stable integration using puromycin treatment (0.5 μg/mL) and propagated as a non-clonal pool. The expression of stem marker genes was validated by immunofluorescence and western blots for rabbit anti-Nanog (Abcam), rabbit anti-Sox2 (Abcam), rabbit anti-Oct4 (Abcam), and mouse anti-SSEA4 (Thermo Fisher).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!