Recombinant april

Manufactured by Merck Group

Sourced in United Kingdom

Recombinant APRIL is a laboratory product manufactured by Merck Group. It is a protein that serves as a core function in experimental research. The detailed technical specifications and intended use of this product are not available in this factual and unbiased format.

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Lab products found in correlation

Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions) Recombinant human april, by R&D Systems (2 mentions) Mab5931, by R&D Systems (2 mentions) Mab5860, by R&D Systems (2 mentions) A6730, by Merck Group (2 mentions) Pd98059, by Merck Group (1 mentions) Ly294002 hydrochloride, by Merck Group (1 mentions) Ly294002 hydrochloride l9908, by Merck Group (1 mentions) Pd98059 p215, by Merck Group (1 mentions)

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APRIL (3 citations)

2 protocols using recombinant april

In Vitro Neuronal Transfection and APRIL Signaling

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After 2 or 6 days in vitro, the neurons were transfected with expression vectors using lipofectamine 2000 (Invitrogen, Paisley, UK) according to the manufacturer's instructions with modifications. Briefly, 1.2 μg of DNA was mixed with 4 μl of lipofectamine. After 20 min, this mixture was added to the cultures. After 3 h, the cultures were then washed with culture medium, at which point recombinant human APRIL was added (R&D Systems, Abingdon, UK) and the neurons were maintained in culture for further 18 h. Chemical inhibitors (PD98059, Sigma-P215, LY294002 hydrochloride, Sigma-L9908 and Akt1/2 kinase inhibitor A6730, Sigma-Aldrich Company, Dorset, UK) were added 2 h before incubation of recombinant APRIL. Function-blocking antibodies (mouse anti-BCMA, MAB5931 and human anti-APRIL, MAB5860, R&D Systems, Abingdon, UK) were added after transfection.

Osório C., Chacón P.J., White M., Kisiswa L., Wyatt S., Rodríguez-Tébar A, & Davies A.M. (2014). Selective regulation of axonal growth from developing hippocampal neurons by tumor necrosis factor superfamily member APRIL. Molecular and Cellular Neurosciences, 59(100), 24-36.

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Neuronal Transfection and APRIL Signaling

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After 2 or 6 days in vitro, the neurons were transfected with expression vectors using lipofectamine 2000 (Invitrogen, Paisley, UK) according to the manufacturer’s instructions with modifications. Briefly, 1.2 μg of DNA was mixed with 4 μl of lipofectamine. After 20 min, this mixture was added to the cultures. After 3 h, the cultures were then washed with culture medium, at which point recombinant human APRIL was added (R&D Systems, Abingdon, UK) and the neurons were maintained in culture for further 18 h. Chemical inhibitors (PD 98.059 P215, LY-294.002 hydrochloride L9908 and Akt1/2 kinase inhibitor A6730, Sigma-Aldrich Company, Dorset, UK) were added 2hr before incubation of recombinant APRIL. Function blocking antibodies (mouse anti-BCMA, MAB5931 and human anti-APRIL, MAB5860, R&D Systems, Abingdon, UK) were added after transfection.

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