Anti ha agarose a2095

Anti-HA-agarose (A2095) and anti-FLAG affinity gel (A2220) were obtained from Sigma. For Western blotting, secondary antibody against mouse (A21057) was purchased from Invitrogen, and secondary antibody against rabbit (611-732-127) was from Rockland. Antibodies against FLAG (F1804), tubulin (T9026), and His tag (H1029) were purchased from Sigma; anti-HA (MMS-101P) was from Covance; anti-USP7 (sc-30164) was from Santa Cruz Biotechnology; anti-myc (2276) was from Cell Signaling Technology; anti-V5 (46-0705) and secondary antibodies for immunofluorescence were from Invitrogen. Anti-TRF1 has been described previously (26 ).

The cDNAs of various human Gα subunits and AGS3 were purchased from the Missouri S&T cDNA Resource Center. HA-tagged mouse Ric-8A was kindly provided by Dr. Yijuang Chern (National Yang-Ming University). Cell culture reagents, including LipofectAMINE PLUS reagents were purchased from Invitrogen (Carlsbad, CA, USA). Anti-Sox-2 (AB5603), anti-β-III tubulin (MAB1637), anti-Par3 (07-330), anti-inscuteable (ABT64) and anti-nestin (MAB5326) antibodies were from Millipore (Darmstadt, Germany). Anti-β-actin (sab5500001) and anti-HA agarose (A2095) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Antisera against Gα_i1 (sc-13533), Gα_i2 (sc-7276), Gα_i3 (sc-262), aPKC (sc-216), AGS3 (sc-33222), Par6 (sc-25525) and GFP (sc-8334) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-NuMA antibody (ab97585) was from Abcam (Cambridge, UK). Anti-GPSM2/LGN (PAB7195) was from Abnova (Taipei City, Taiwan). Anti-GST (#2625) antibody was purchased from Cell Signaling Technology (Danvers, MA, USA). GDPβS and GTPγS were from Calbiochem (San Diego, CA, USA). Protein G agarose was purchased from Roche Diagnostics (Indianapolis, IN, USA).