Fluorescent probes of mito tracker deep red

Manufactured by Thermo Fisher Scientific

Fluorescent Probes of Mito-tracker deep red is a mitochondrial stain that selectively accumulates in active mitochondria. It exhibits far-red fluorescence upon binding to the mitochondrial membrane.

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Fluorescent probes (34 citations)

2 protocols using fluorescent probes of mito tracker deep red

Antibody Validation for Western Blotting

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For Western blotting, antibodies of anti-Ki67 (Abcam plc, ab15580), anti-Hsp60 (Abcam plc, ab46798), anti-β-actin (Cell Signaling Technology, 4970S), anti-cleaved caspase-3 (Cell Signaling Technology, 9661S), anti-Nur77 (Cell Signaling Technology, 3960S), anti-Myc (9E10) (Santa Cruz Biotechnology, Sc-40), anti-PCNA (Santa Cruz Biotechnology, sc-7907), anti-α-Tubulin (Santa Cruz Biotechnology, sc-8035), anti-Bcl-2 (Santa Cruz Biotechnology, sc-783), anti-Bcl-2 antibody (Abmart, T40056F), anti-PARP (Santa Cruz Biotechnology, sc-7150) were purchased and all the above antibodies were used in the dilution ratio of 1:1000. For immunoprecipitation, anti-Flag (Sigma–Aldrich, F1804, dilution: 1:100), anti-Bcl-2 (Santa Cruz Biotechnology, sc-65392, dilution: 1:50) was utilized. For immunofluorescence staining, Fluorescent Probes of Mito-tracker deep red (ThermoFisher Scientific, M22426, dilution: 1:20,000), JC-1 Probe (Thermo Fisher Scientific, T3168, 1:500) and Mito-SOX Red Mitochondrial Superoxide Indicator (Thermo Fisher Scientific, M36008, dilution: 1:3000) were utilized.

Chen X., Gao M., Xia Y., Wang X., Qin J., He H., Liu W., Zhang X., Peng S., Zeng Z., Su Y, & Zhang X. (2023). Phase separation of Nur77 mediates XS561-induced apoptosis by promoting the formation of Nur77/Bcl-2 condensates. Acta Pharmaceutica Sinica. B, 14(3), 1204-1221.

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Antibody Validation for Western Blotting

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