Rat anti lamp1 1d4b

Primary antibodies were: mouse monoclonal antibodies against Myc clone 9E10 (Sigma), Flotillin-2 (BD Biosciences), α-Synuclein (Invitrogen), Alix (BD Biosciences), TSG101 (GeneTex Inc., Irvine, CA, USA), CD63 (BD Biosciences), 6E10 anti-APP (Signet), GAPDH clone 6C5 (Abcam), rabbit anti-Glutamate Receptor GluR2/3 (Chemicon), rabbit anti-Glutamate Receptor GluR1 (Chemicon), rabbit anti-Calnexin (StressGene), rabbit anti-GFP (Invitrogen), rabbit anti-Integrin β5 (Millipore), rabbit anti-UBC9 (Santa Cruz), and rat anti-LAMP1 (1D4B) (Santa Cruz). SUMO-2 antibody was kindly provided by F. Melchior (Heidelberg, ZMBH, Germany) [5 (link)]. Secondary antibodies were obtained from Dianova and Invitrogen.

Rabbit anti-HA antibody (600-401-384) was purchased from Rockland Immunochemicals (Gilbertsville, PA, USA). Mouse anti-HA antibody (16B12) was purchased from Biolegend (San Diego, CA, USA). Mouse anti-calnexin (AF18), EEA1 (E-8), TGN38 (B-6), and rat anti-LAMP1 (1D4B) antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Rabbit anti-p62 antibody (PM045) was purchased from MBL (Nagoya, Japan). Mouse anti-β-actin antibody (A5441) and horseradish peroxidase (HRP)-conjugated rabbit anti-mouse IgG antibody (A9044) were purchased from Sigma Aldrich (St. Louis, MO, USA). HRP-conjugated donkey anti-rabbit IgG antibody (711-035-152) was purchased from Jackson ImmunoReseach Laboratories (West Grove, PA, USA). Alexa Fluor 488 (A11008)- and 647 (A21244)-conjugated goat anti-rabbit IgG antibody and Alexa Fluor 647-conjugated anti-mouse IgG antibody (A21235) were purchased from Invitrogen (Carlsbad, CA, USA).