Aeroset autoanalyzer

The serum level of hs-CRP was measured immediately with an Aeroset autoanalyzer (Abbott, Maidenhead, UK) using a spectrophotometric commercial kit (Scil Diagnostics GmbH, Viernheim, Germany).

BPs was measured using the first and fifth phases of the Korotkoff sound, by a cuff sphygmomanometer. Body mass index (BMI) was calculated as the weight divided by the height squared (kg/m2). The body surface area (BSA, m2) of each patient and other clinical characteristics were also recorded. Blood samples were drawn from the antecubital vein as soon as patients were admitted to ED. All of the measurements were performed within 1 h of venipuncture to prevent in-vitro platelet activation. Blood specimens were collected into ethylenediaminetetraacetic acid (EDTA) containing tubes and analyzed with the aid of a Cell-Dyn 4000 cell counter (Abbott; North Chicago, IL, USA) using commercial kits (Abbott, USA). The normal MPV was accepted as 6.8–10.8 fL. NLR was calculated from the levels of neutrophils and lymphocytes, manually. The levels of plasma triglycerides, low-density lipoprotein cholesterol, high-density lipoprotein cholesterol, creatinine, glucose, hemoglobin, and the creatine-kinase MB isoenzyme were measured using commercial kits. Hs-CRP levels were measured with the aid of an Aeroset autoanalyzer using a commercial spectrophotometric kit (Abbott, USA).

Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activity, total bilirubin (TBil), total bile acid (TBA), and albumin (Alb) content were assayed using kits from Shanghai Institute of Biological Products (Shanghai, China). Levels of alkaline phosphatase (ALP) and γ-glutamyl transferase (GGT) were assessed using kits from Nanjing Jianchen Biotech Institute (Nanjing, China). TBA was analyzed with an Abbott Aeroset Autoanalyzer (Abbott Park, IL).