Thermo scientific vanquish uplc

For qualitative analysis, a Thermo Scientific Q Exactive Orbitrap HRMS (Thermo Fisher Scientific, Massachusetts, United States) was connected to a Thermo Scientific Vanquish UPLC (Thermo Fisher Scientific, Massachusetts, United States). Chromatographic separation was achieved on a Thermo Scientific Accucore C₁₈ (3 × 100 mm, 2.6 μm) in a thermostatically controlled column compartment (30 °C). The aqueous and organic mobile phases used were acetonitrile (A) and 0.1% phosphoric acid aqueous solution (B), respectively. The gradient elution program was set up as follows: 0–20 min, 12–25% A; 20.1–30 min, 25–28% A; 30.1–32 min, 28–40% A; 32.1–40 min, 40–50% A; 40.1–60 min, 50% A; 60.1–65 min, 12% A. The flow rate was set as 0.3 ml/min, and 2 μl of the extraction was injected into the UPLC system. The instrument was operated in the positive ion mode to perform full-scan analysis over an m/z range of 100–1,500. The optimized parameters were set as follows: a sheath gas flow rate of 35 L/min; a spray voltage of 3000 V; a capillary temperature of 320 V; an aux gas flow rate of 10.00 L/min; a max spray current of 100 A; a probe heater temperature of 350 C; and an S-lens RF level of 50.00%.

For qualitative analysis, a Thermo Scientific Q Exactive Orbitrap HRMS (Thermo Fisher Scientific, Massachusetts, USA) was connected to a Thermo Scientific Vanquish UPLC (Thermo Fisher Scientific, Massachusetts, USA). Chromatographic separation was achieved on a Thermo Scientific™ Accucore™ C18 (3 × 100 mm, 2.6 μm) in a thermostatically controlled column compartment (30 °C). The aqueous and organic mobile phases used were 0.1% formic acid in water (A) and methyl alcohol (B), respectively. A gradient elution system was set up as follows: 0–20 min, 5–80% B; 20–30 min, 80–95% B; 30.1–35 min, 95–5% B. The flow rate was 0.3 mL/min, and 2 μL of the extraction was injected to the LC system. The instrument was operated in positive ion mode to perform full-scan analysis over an m/z range of 100–1500. And the optimized parameters were set as follows: the sheath gas flow rate – 35 L/min; spray voltage – 3000 V; capillary temperature – 320 V; aux gas flow rate – 10.00 L/min; max spray current – 100A; probe heater temperature – 350 °C; S-lens RF level – 50.00%.