Mouse α myc

Manufactured by Santa Cruz Biotechnology

Mouse α-myc is a primary antibody that recognizes the c-myc protein, a basic helix-loop-helix leucine zipper transcription factor that plays a role in cell cycle progression, apoptosis, and cellular transformation. This antibody can be used in various applications such as Western blotting, immunoprecipitation, and immunohistochemistry to detect the c-myc protein in mouse samples.

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Lab products found in correlation

Rpn2209, by Cytiva (1 mentions) Horseradish peroxidase coupled secondary antibody, by Bio-Rad (1 mentions) Mouse α gfp, by Roche (1 mentions) Rabbit α flag, by Merck Group (1 mentions) Mouse monoclonal α flag m2, by Merck Group (1 mentions)

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α-Myc (12 citations)

2 protocols using mouse α myc

Western Blot Analysis of Protein Samples

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Protein samples were run according to standard protein separation procedures, using SDS-PAGE. The following primary antibodies were used: mouse α-GFP (1:2000; Roche Cat# 11814460001, RRID:AB_390913), mouse α-myc (1:2000; Santa Cruz Biotechnology Cat# sc-40, RRID:AB_627268), rat α-RBP10 (1/500; RRID:AB_2890154), rabbit α-aldolase (1/2000; RRID:AB_2890155). We used horseradish peroxidase coupled secondary antibodies (1:2000; Bio-Rad Cat# 170–6516, RRID:AB_11125547; Bio-Rad Cat# 170–6515, RRID:AB_11125142). Blots were developed using an enhanced chemiluminescence kit (Amersham; RPN2209) according to the manufacturer’s instructions. Densitometry was performed using Fiji v. 2.0.0.

Melo do Nascimento L., Egler F., Arnold K., Papavasiliou N., Clayton C, & Erben E. (2021). Functional insights from a surface antigen mRNA-bound proteome. eLife, 10, e68136.

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Immunoblotting with Epitope-Tagged Proteins

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The antibodies used were mouse α-Myc (Santa Cruz Biotechnology), mouse monoclonal α-FLAG M2 (Sigma-Aldrich), rabbit α-FLAG (Sigma-Aldrich), and α-V5-HRP conjugate (Invitrogen).

Halabi S., Sekine E., Verstak B., Gay N.J, & Moncrieffe M.C. (2016). Structure of the Toll/Interleukin-1 Receptor (TIR) Domain of the B-cell Adaptor That Links Phosphoinositide Metabolism with the Negative Regulation of the Toll-like Receptor (TLR) Signalosome. The Journal of Biological Chemistry, 292(2), 652-660.

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