Mpt 2 autotitrator

Manufactured by Malvern Panalytical

Sourced in United Kingdom

The MPT-2 Autotitrator is a robust and precise automated titration system designed for a wide range of applications. It performs accurate and repeatable titrations, including acid-base, complexometric, and redox titrations, for various sample types. The MPT-2 Autotitrator features a user-friendly interface, multiple burette options, and advanced data analysis capabilities, making it a versatile choice for analytical laboratories.

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Lab products found in correlation

Zetasizer nano z, by Malvern Panalytical (4 mentions) Zetasizer nano, by Malvern Panalytical (1 mentions) V 630 spectrophotometer, by Jasco (1 mentions) Jem 2100 hrtem, by JEOL (1 mentions) Fp 6600 spectrofluorometer, by Jasco (1 mentions) Zetasizer nano zse, by Malvern Panalytical (1 mentions) Malvern zetasizer nano z, by Malvern Panalytical (1 mentions) Direct q3, by Merck Group (1 mentions) Dts 1070 disposable folded capillary cells, by Malvern Panalytical (1 mentions) Ls230, by Beckman Coulter (1 mentions)

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MPT-2 (16 citations) Autotitrator (2 citations)

9 protocols using mpt 2 autotitrator

Characterization of Copper Nanoclusters

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Emission
spectral studies were monitored
by a JASCO FP-6600 spectrofluorometer with excitation wavelength at
380 nm in the emission mode. Both the emission and excitation slit
widths were kept at 5 nm. Absorption spectra were recorded from 800
to 200 nm by a JASCO V-630 spectrophotometer with 1.0 cm quartz cells.
Nanostructure and morphology of NCs were verified by JEOL JEM 2100
HR-TEM operating at 200 kV. DLS information was evidenced to determine
the average size (hydrodynamic diameter) of CuNCs. DLS analysis results
were acquired by Malvern Zetasizer Nano. Zeta potential experiments
were recorded by Zetasizer Nano, ZS with 633 nm He–Ne laser,
equipped with a MPT-2 autotitrator (Malvern, UK). Fluorescence lifetime
experiments were performed on Horiba Scientific equipped with an NL-C2
pulsed diode excitation source of 380 nm.

Rajamanikandan R, & Ilanchelian M. (2018). Fluorescence Sensing Approach for High Specific Detection of 2,4,6-Trinitrophenol Using Bright Cyan Blue Color-Emittive Poly(vinylpyrrolidone)-Supported Copper Nanoclusters as a Fluorophore. ACS Omega, 3(12), 18251-18257.

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Zeta Potential Titration of Liposomes

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Titrations with 0.1 M HCl were performed using an MPT-2 autotitrator (Malvern Instruments Ltd., Malvern, UK) in order to determine the zeta potential of liposomes or native-like envelope trimers as a function of pH. Liposomes were diluted with a low-ionic-strength buffer (5 mM BB sucrose pH 8.5 w/300 mM sucrose) to a lipid concentration of 250 µM, whereas native-like HIV-1 envelope trimers were diluted to a concentration of 83 nM (30 µg/mL).

Suleiman E., Mayer J., Lehner E., Kohlhauser B., Katholnig A., Batzoni M., Damm D., Temchura V., Wagner A., Überla K, & Vorauer-Uhl K. (2020). Conjugation of Native-Like HIV-1 Envelope Trimers onto Liposomes Using EDC/Sulfo-NHS Chemistry: Requirements and Limitations. Pharmaceutics, 12(10), 979.

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Zeta Potential and DLS Analysis of Polysaccharides and Anthocyanins

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The zeta potential and DLS measurements were performed using a Zetasizer Nano ZS (Zetasizer Nano ZS, Malvern Instruments Inc., Malvern, UK). All the zeta potential (n = 3) and DLS measurements (n = 6) were carried out at 25 ℃. Zeta potential was read from pH 12.00 to 1.00 in 1.00-unit increments with a 0.20 pH unit tolerance and three readings per pH value using an MPT-2 Autotitrator (Malvern Instruments Inc., Malvern, UK). The samples (polysaccharides: 5 mg/mL; anthocyanins: 0.25 mg/mL) were suspended in water for zeta potential and DLS readings [25 (link)]. All measurements for zeta potential and DLS were conducted in triplicate and the averages were reported.

Fu W., Li S., Helmick H., Hamaker B.R., Kokini J.L, & Reddivari L. (2023). Complexation with Polysaccharides Enhances the Stability of Isolated Anthocyanins. Foods, 12(9), 1846.

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Organic and Aqueous Self-Assembly of Nanoparticles

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For organic solvent-mediated self-assembly, TA, DSPE-PEG2k, PEG10k, and F68 were first dissolved in DMSO at a concentration of 20 mg/mL. Afterwards, TA and one of the three polymers were mixed at a weight ratio of 1:1. Finally, the mixture in DMSO was added dropwise into 10 mM PBS at a final concentration of 0.1 mg/mL to obtain the self-assembled nanoparticles. For aqueous self-assembly, TA, DSPE-PEG2k, PEG10k, and F68 were first dissolved in DI water at a concentration of 10 mg/mL. Then, one of the three polymers was added to 10 mM PBS (pH 8.5-9.0) at a final concentration of 0.05 mg/mL. Finally, the same amount of TA was added to the solution and the pH was adjusted to approximately 7 using 0.01M HCl. Hydrodynamic size, zeta potential, scattering intensity and pH titration measurements by DLS were performed with a Zetasizer Nano ZSE equipped with MPT-2 autotitrator (Malvern Instruments, Ltd.).

Wang X., Yan J., Wang L., Pan D., Xu Y., Wang F., Sheng J., Li X, & Yang M. (2020). Oral delivery of anti-TNF antibody shielded by natural polyphenol-mediated supramolecular assembly for inflammatory bowel disease therapy. Theranostics, 10(23), 10808-10822.

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Polymer Network Surface Zeta-Potential Analysis

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The effective surface ζ-potential of the polymer networks was measured using a Malvern Zetasizer NanoZS (Malvern Instruments Corp., Malvern, UK) operating with a 633 nm laser source equipped with a MPT-2 Autotitrator. Measurements of ζ-potential as a function of pH were conducted by resuspending lyophilized particles in 5 mM phosphate buffer at 0.5 mg/mL. The suspension pH was adjusted from pH 4.5 to 8.5 using 1 N NaOH. Electrophoretic light scattering measurements of the surface ζ- potential were collected at 25 °C.

Caldorera-Moore M., Vela Ramirez J.E, & Peppas N.A. (2019). Transport and Delivery of Interferon-α Through Epithelial Tight Junctions via pH-Responsive Poly(Methacrylic Acid Grafted Ethylene Glycol) Nanoparticles. Journal of drug targeting, 27(5-6), 582-589.

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Zeta Potential Analysis of Diluted Samples

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Samples extracted under different conditions were diluted in Milli-Q water (Direct-Q3, Millipore, USA) to a concentration of 0.01% (w/w) and placed in the measuring chamber of a microelectrophoresis system (Zetasizer Nano-ZS, Malvern Instruments Ltd, UK) equipped with an MPT-2 Autotitrator (Malvern Instruments, Herrenberg, Germany). Zeta potential was determined as a function of pH, between 3 and 9. The Smoluchowski model was used to convert the electrophoretic mobility measurement into zeta potential values. The samples were measured in triplicate at 25 °C.

Miranda C.G., Speranza P., Kurozawa L.E, & Kawazoe Sato A.C. (2022). Lentil protein: impact of different extraction methods on structural and functional properties. Heliyon, 8(11), e11775.

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Zeta Potential Measurement of Proteins

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The ζ-potential measurements were done using a Zetasizer Nano ZS (Malvern Instruments, Ltd., Malvern, Worcestershire, UK) coupled with an autotitrator (MPT-2, Autotitrator, Malvern Instruments Ltd.) to determine the surface charge of the protein as a function of the pH. To adjust the pH of the solution (from 2.0 -8.0), 0.1 M NaOH and 0.1 M HNO3 were used. Measurements were done at 25 °C. Protein solutions were prepared at 0.2% final concentration at pH 7.0 using 0.05 M imidazole buffer and left to dissolve overnight on a magnetic stirrer at room temperature. For measurements, DTS-1070 disposable folded capillary cells (Malvern Instruments, Ltd.) were used. ζ-Potential was calculated by the software with the instrument using Henry's equation:
where η is the solution viscosity (Pa s -1 ), µ is the electrophoretic mobility (V Pa -1 s -1 ), ε is the medium dielectric constant (ε = 80) and 𝑓𝑓(𝜅𝜅𝑅𝑅 𝐻𝐻 ) is the Henry's function. κ is the Debye length (nm) and RH is the particle radius (nm) and the RH is accepted as 1.5 due to the aqueous medium used for the analysis, referred to as the Smoluchowski approximation. Isoelectric point (pI) was determined by the intersection on the x-axe at 0 mV. The values between -2.2 and +1.8 mV (dotted lines) were not possible to calculate properly due to the presence of aggregates.

Subaşı B.G., Casanova F., Çapanoğlu E., Ajalloueian F., Sloth J.J., & Mohammadifar M.A. (2020). Protein extracts from de-oiled sunflower cake: Structural, physico-chemical and functional properties after removal of phenolics. Food Bioscience, 38, 100749-100749.

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Potentiometric Titration of GC and EAGC

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GC and EAGC hydrochloride salts [prepared by adjusting the pH of the initial solutions in water to pH = 2 with HCL (0.1M)] at a concentration of 2 mg mL -1 were titrated with NaOH (0.05M).
Under continuous stirring, the titrant was added drop wise. During the titration, the volume of NaOH added and pH values of polymer solutions were recorded using a MPT-2 Autotitrator, (Malvern Instruments, Malvern, UK).
The buffer capacity (C) is defined as the percentage of amino groups that become protonated in the pH range 5.1 to 7.4, and may be calculated using equation 1:
where V = volume of sodium hydroxide used to raise the pH to 7.4, M = number of moles of sodium hydroxide per L, N = number of protonable nitrogen atoms contained in the known amount of EAGC or GC being titrated (Lin et al., 2008) .

Simão Carlos M.I., Zheng K., Garrett N., Arifin N., Workman D.G., Kubajewska I., Halwani A.A., Moger J., Zhang Q., Schätzlein A.G, & Uchegbu I.F. (2017). Limiting the level of tertiary amines on polyamines leads to biocompatible nucleic acid vectors. International journal of pharmaceutics, 526(1-2).

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Characterization of Hydroxyapatite Nanodispersions

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Particle size and zeta potential of the prepared nanodispersions (C HAp = 1.6 g/l and 6.4 g/l) were determined by dynamic and Temperature and pH were also monitored during the preparation process. The effect of pH on the zeta potential was studied by performing titrations using the MPT-2 Autotitrator, an external device connected to the Zetasizer Nano ZS from Malvern Instruments. Moreover, dispersions with higher n-HAp concentration (C HAp = 20 g/l), alone and in the presence of chitosan, were characterized by laser diffraction spectroscopy using a Coulter LS-230.

Ruphuy G., Weide T., Lopes J.C.B., Dias M.M, & Barreiro M.F. (2018). Preparation of nano-hydroxyapatite/chitosan aqueous dispersions: From lab scale to continuous production using an innovative static mixer. Carbohydrate polymers, 202.

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