Hyrax m55

Manufactured by Zeiss

Sourced in Germany

The Hyrax M55 is a versatile scanning electron microscope (SEM) designed for a wide range of applications. It features a high-resolution imaging system and advanced analytical capabilities. The Hyrax M55 provides detailed, high-quality images and data to support scientific research and industrial applications.

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Lab products found in correlation

Axio scope a1, by Zeiss (1 mentions) Hematoxylin, by Carl Roth (1 mentions) Nb110 55674, by Novus Biologicals (1 mentions) Vulcan fast red ap substrate solution, by Biocare Medical (1 mentions) Stp 120 spin tissue processor, by Thermo Fisher Scientific (1 mentions) Appliclear, by AppliChem (1 mentions) Axio scope a1 microscope, by Zeiss (1 mentions) Paraformaldehyde (pfa), by Merck Group (1 mentions) Hematoxylin and eosin, by Avantor (1 mentions) Hv f202fcl, by Hitachi (1 mentions) Axio scan z1, by Zeiss (1 mentions) Polysine adhesion slide, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Hyrax M55 rotary microtome (3 citations) Microtome (Hyrax M55; (2 citations)

8 protocols using hyrax m55

Quantitative Analysis of Adipose Tissue

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Adult mice were euthanized by CO₂. Adipose tissues were excised and stored in aliquots at −80 °C. The samples from visceral and subcutaneous adipose tissue were rapidly thawed using a water bath and then fixed in 4% paraformaldehyde (Sigma Aldrich) at pH 7.4. The tissue processing was performed according to the standard protocol in a tissue processor (STP 120; Microm). The samples were embedded in paraffin and cut into 5 um thin sections on a Hyrax M55 (Zeiss). Sections were de-paraffinized and stained with haematoxylin and eosin on a Varistain 24–4 (Thermo Scientific) before mounting and drying. Images were obtained using an Axioscope A.1 (Zeiss). The analysis of lipid droplet sizes was performed using CellProfiler (www.cellprofiler.org).

Sarker G., Litwan K., Kastli R, & Peleg-Raibstein D. (2019). Maternal overnutrition during critical developmental periods leads to different health adversities in the offspring: relevance of obesity, addiction and schizophrenia. Scientific Reports, 9, 17322.

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Histological Analysis of Healthy and IPF Lung Tissue

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Resected human lung tissue and explant material was obtained from the bioarchive at the Comprehensive Pneumology Center (CPC). Biopsies were obtained from 6 healthy donors and 5 IPF patients (UIP pattern, mean age: 57,6 ± 3,25, 3 males, 2 females). All participants gave written informed consent and the study was approved by the local ethics committee of Ludwig-Maximilians University of Munich, Germany (333-10). For staining, human lung tissue was fixed in 4 % PFA prior to paraffin embedding. The 4 μm-sections were prepared with a microtome (Hyrax M 55, Zeiss) and mounted on Superfrost slides.

Smirnova N.F., Schamberger A.C., Nayakanti S., Hatz R., Behr J, & Eickelberg O. (2016). Detection and quantification of epithelial progenitor cell populations in human healthy and IPF lungs. Respiratory Research, 17, 83.

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Immunohistochemistry of Lung Tumor Samples

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Lung tumor specimens from human and Kras^LA2 mutant mice were placed in 4% (w/v) paraformaldehyde (PFA) overnight at 4°C and processed for paraffin embedding. 3 μm thick paraffin sections were sliced with the microtome (Zeiss Hyrax M 55) and placed on superfrost plus adhesion slides. De-paraffinized sections were subjected to quenching of endogenous peroxidase activity using a mixture of methanol/H₂O₂ for 20 min, followed by antigen retrieval in a de-cloaking chamber. From this step on, the slides were washed with tris-buffered saline with Tween-20 (TBST, 20 mM Tris, 0.8% NaCl, 0.02% Tween-20, pH 7.6 adjusted with HCl) after each incubation with the reagents throughout the procedure. The sections were incubated first with Rodent Block M (Zytomed Systems) for 30 min and then with the primary antibody, i.e., EGFR (Cell Signaling, D38B1 for human, Abcam, ab52894 for mouse), CCR2 (Novus Biologicals, NB110-55674), or IgG control for 1 h. The cuts were then incubated with Rabbit on Rodent AP-Polymer for 30 min, which was followed by Vulcan Fast Red AP substrate solution (both Biocare Medical) incubation for 10-15 min. Sections were counterstained with hematoxylin (Carl Roth) and dehydrated respectively in consecutively grading ethanol and xylene (both Appli-Chem) incubations. Dried slides were mounted in Entellan and visualized with a slide scanner.

Bölükbas D.A., Datz S., Meyer-Schwickerath C., Morrone C., Doryab A., Gößl D., Vreka M., Yang L., Argyo C., van Rijt S.H., Lindner M., Eickelberg O., Stoeger T., Schmid O., Lindstedt S., Stathopoulos G.T., Bein T., Wagner D.E, & Meiners S. (2020). Organ-restricted vascular delivery of nanoparticles for lung cancer therapy. Advanced therapeutics, 3(7), 2000017.

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Fixation and Embedding Protocol for Jejunum Tissue

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Two cm pieces of jejunum were collected into tissue processing/embedding cassettes and fixed overnight in 4% paraformaldehyde at 4 °C. They were transferred to 65% ethanol in an automatic paraffin-embedding processor (STP 120 spin Tissue Processor, Thermo Fischer, Waltham, MA, USA). The tissues were embedded in paraffin blocks with an automatic embedding system (Automatic Embedding System Bio-optica, Milano, Italy). Paraffin sections were cut with a motorized rotary microtome (Hyrax M55, Zeiss, Oberkochen, Germany) and disposable microtome razor blades (S-22 blades, Feather, Osaka, Japan).

Ramachandran D., Clara R., Fedele S., Hu J., Lackzo E., Huang J.Y., Verdin E., Langhans W, & Mansouri A. (2017). Intestinal SIRT3 overexpression in mice improves whole body glucose homeostasis independent of body weight. Molecular Metabolism, 6(10), 1264-1273.

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Paraffin Embedding and Sectioning of Plant Samples

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Tissue blocks (ca. 3 mm thick) comprising the fruit skin and some subtending parenchyma cells were excised from the treated or the control patches of the fixed fruit using a scalpel. The blocks were rinsed in distilled water and immersed in 70% (v/v) aqueous ethanol for 16 h. The blocks were then dehydrated in an ascending series of ethanol (80%, 90% and 96% v/v; 30 min each) under a partial vacuum (pressure 10.8 kPa). Subsequently, the blocks were transferred to 100% isopropanol for 40 min (twice) and a xylene substitute (AppliClear; AppliChem, Münster, Germany) for 40 min (twice) to displace the ethanol in the tissues, under the same partial vacuum. The dehydrated blocks were then infiltrated with a 1:1 (v/v) paraffin/xylene substitute mixture (Carl Roth) for 40 min (once) and paraffin alone for 40 min (twice). Finally, the blocks were embedded in paraffin. The paraffin blocks so obtained were cooled and stored at 4 °C pending later sectioning.
Thin sections (10 µm) were cut using a rotary microtome (Hyrax M 55, Zeiss, Germany). Sections were transferred to microscope slides, dried in an oven for 16 h at 38 °C and rehydrated as follows: xylene substitute (2 × 10 min); descending series of ethanol (96%, 80%, 70% and 60% for 10 min each) and finally for 2 × 5 min in distilled water.

Chen Y.H., Straube J., Khanal B.P., Knoche M, & Debener T. (2020). Russeting in Apple Is Initiated After Exposure to Moisture Ends—I. Histological Evidence. Plants, 9(10), 1293.

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Histological Analysis of Adipose and Liver Tissues

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For stainings with hematoxylin and eosin (H&E) and oil red O (ORO) adipose and liver tissues were fixed in 4% PFA (Sigma–Aldrich) and processed in a STP 120 (Microm) according to the standard protocol. Paraffin-embedded samples were cut at 5 μm on a Hyrax M55 (Zeiss), deparaffinized and stained on a Varistain 24-4 (Shandon) for adipose tissue or ORO (Sigma–Aldrich, cat # 00625) for liver [54] (link). Pictures were obtained with an Axioscope A.1 microscope (Zeiss). H&E and ORO images were quantified for lipid fraction and ORO area using ImageJ software.

Lee S.J., Sanchez-Watts G., Krieger J.P., Pignalosa A., Norell P.N., Cortella A., Pettersen K.G., Vrdoljak D., Hayes M.R., Kanoski S.E., Langhans W, & Watts A.G. (2018). Loss of dorsomedial hypothalamic GLP-1 signaling reduces BAT thermogenesis and increases adiposity. Molecular Metabolism, 11, 33-46.

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Histopathological Analysis of Lung Tumors

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Lungs were perfused with saline and fixed using 4% paraformaldehyde overnight. The lungs were dehydrated and embedded in paraffin blocks. 5 µm histology cuts were done using a Zeiss Hyrax M55 rotary microtome (Switzerland). Staining was performed using Hematoxylin and Eosin (VWR). Imaging was performed on the Zeiss Axio Scan Z.1 slide scanner (20× objective power, 0.8 NA and a 8 bit Hitachi HV-F202FCL color camera (1600 × 1200 pixels, 2 MP, pixel size 4.4 μm). CZI files were exported as a TIFF file in order for the tumor tissue to be annotated manually by using ImageScope 12.4.3.5008 software (LEICA, Wetzlar, Germany). Lung sections were annotated for tumor tissue and total lung tissue, excluding large bronchi and other non-lung tissues.

Vasilikos L., Hänggi K., Spilgies L.M., Kisele S., Rufli S, & Wong W.W. (2021). Loss of cIAP1 in Endothelial Cells Limits Metastatic Extravasation through Tumor-Derived Lymphotoxin Alpha. Cancers, 13(4), 599.

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Tissue Microarray Preparation for Immunohistochemistry

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Manual-Tissue-Arraying instrument (MTA Booster, Version 01, Alphelys, France).
To avoid random staining events, three tumor tissue cylinders and two urothelial cylinders as control were obtained. For obtaining tumor tissues, hollow needles with a diameter of 0.6 mm were used. To ensure that urothelial tissue was obtained, hollow needles with a diameter of 1.0 mm were used. A total of nine TMAs were created. The TMAs were placed in an incubator at 40°C to embed the tissue cylinders into paraffin. A motorized Zeiss Hyrax M55 rotation microtome (Zeiss, Oberkochen, Germany) was used for cutting 4 μm thick slices. After smoothing in a water bath, slices were fixed onto Polysine adhesion slides (Thermo Scientific, Waltham, MA, USA). Eleven slices were prepared on 9 TMAs to be probed with antibodies against 11 eIFs.
Patient collective. For the study, tumor tissue samples from 107 patients with UC were examined immunohistochemically (tumor collective). Of these 107 patient samples, 76 contained urothelial NNT and were suitable as a control collective for the expression analyses. Figure 1 shows the distribution of the grades among the patients' collective, and Figure 2 shows the distribution of the T stages in the patient cohort.

Luzha J., Nass N., Czapiewski P., Schroeder N., Kalinski T., Schostak M., Schatz C., Jandrig B, & Haybaeck J. (2023). Expression of Eukaryotic Translation Initiation Factors in the Urothelial Carcinoma of the Bladder. Anticancer research, 43(4).

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