Total RNA was extracted from cultured cells or E18 mouse forebrain using the Hybrid-RTM RNA extraction kit (GeneAll, 305-101) and reverse-transcribed with SuperScriptTM III First-Strand Synthesis System (Invitrogen, 18080051). The 1st cDNA was amplified by polymerase chain reaction (PCR) using PowerSYBR® Green PCR MasterMix (ABI, 4367659) on the StepOnePlus Real-Time PCR System (ABI). Mouse GAPDH primers were used for the normalization of RNA expression. The sequences of all primers used in this study are provided in Table 1 .
Hybrid rtm rna extraction kit
Manufactured by GeneAll
The Hybrid-RTM RNA extraction kit is a laboratory equipment product designed for the extraction and purification of RNA from various biological samples. It utilizes a hybrid extraction method to efficiently isolate high-quality RNA for downstream applications.
Automatically generated - may contain errors
2 protocols using hybrid rtm rna extraction kit
1
Quantitative Gene Expression Analysis
2
RNA-seq Library Preparation and Sequencing
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total RNA was extracted using a Hybrid-RTM RNA extraction kit (GeneAll, 305-101), according to the manufacturer’s instructions, and RNA quality was confirmed using a Bioanalyzer 2100 system (Agilent Technologies). To prepare the RNA libraries, 1 μg of total RNA was processed using the TruSeq Stranded mRNA LT Sample Prep Kit (Illumina, RS-122-9005DOC). The libraries were sequenced using Illumina NovaSeq and low-quality bases and adapter sequences were removed from the raw data using Trimmomatic (v.0.36). The clean reads were aligned to the human genome (hg38) using HISAT2 (v.2.2.1) with default parameters, and gene expression levels were quantified using a unique alignment to Cufflinks (v.2.2.1).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!