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2 protocols using 7 dehydrocholesterol 7 dhc

1

Quantification of Vitamin D5 Isomer

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Authentic vitamin D532 (link) ((5Z,7E)-9,10-secostigmasta-5,7,10(19)-trien-3β-ol, CAS 71761-06-3, 85% chemical purity) was purchased from BOC Sciences, Shirley, NY 11967, USA. Sitosterol (Purity ≥ 95%) and 7-dehydrocholesterol (7-DHC) (Purity > 98%), were purchased from Sigma-Aldrich (Steinheim, Germany) and used as standards. Stock solutions of the standards were prepared in benzene (Fluka) and stored at −20 °C.
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2

Quantitative Cholesterol Profiling Protocol

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The cholesterol-d7 (>99%) reference standards were purchased from Avanti Polar Lipids (Alabaster, AL, USA). Desmosterol (>84%), 7-dehydrocholesterol (7-DHC, >95%), 7-hydroxycholesterol (7-OHC, >95%), cholesterol (>99%), lanosterol (>93%), stigmasterol (95%), campesterol (65%), ammonium acetate, butylated hydroxytoluene (BHT), and methyl-tert-butyl ether (MTBE) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Cholesteryl ester 20:4 (>98%) and cholesteryl ester 14:0 (>98%) were purchased from Cayman Chemical Company (Ann Arbor, MI, USA). Water, methanol, chloroform, and isopropanol were liquid chromatography–mass spectrometry (LC-MS) grade (Fisher Scientific Co., Pittsburgh, PA, USA).
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