Mir 130b mimic

Manufactured by GenePharma

Sourced in China

MiR-130b mimic is a synthetic molecule designed to mimic the function of the naturally occurring microRNA (miRNA) miR-130b. miRNAs are small, non-coding RNA molecules that play a crucial role in gene expression regulation. The core function of the MiR-130b mimic is to introduce and facilitate the activity of miR-130b in biological systems, enabling researchers to investigate its biological functions and potential applications.

Automatically generated - may contain errors

Lab products found in correlation

Hiperfect transfection reagent, by Qiagen (1 mentions) Mirna mimic, by GenePharma (1 mentions) Oligofectamine, by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Mir 130b inhibitor, by GenePharma (1 mentions) Si pparγ, by GenePharma (1 mentions)

Spelling variants of this product

MiR-mimics (3 citations) MiR-130b-3p mimic (2 citations)

2 protocols using mir 130b mimic

Downregulation of PPARγ and VEGF-A by miR-130b

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MiR-130b inhibitor (anti-M), miR-130b mimic, or the appropriate negative controls of miRNA inhibitor (anti-MC) and miRNA mimic were purchased from GenePharma (Shanghai, China). Anti-M and anti-MC were transfected at a final concentration of 50–100 nM in the cells using HiPerFect Transfection Reagent (Qiagen, Hilden, Germany) according to the manufacturer’s recommendations. Expression of PPARγ and VEGF-A were knocked down with small interfering RNA (siRNA) duplexes using Oligofectamine (Invitrogen, Carlsbad, CA). The target sequences for PPARγ and VEGF-A mRNA were shown in Table 2. Non-targeting siRNA pool (D-001206-13-05; Dharmacon, Fisher Scientific, Pittsburgh, PA) was used as a negative control. Cells were harvested 72 hours post transfection for analysis.Table 2

Target sequences for PPARγ and VEGF-A mRNA

Genes	Target sequences
PPARγ	5’-AAUAUGACCUGAAGCUCCAAGAAUAAG-3’
VEGF-A	5’-TGCTGTGAAGATGTACTCTATCTCGTGTTTTGGCCACTGACTGACACGAGATAGTACATCTTCA-3’

Tian J., Hu L., Li X., Geng J., Dai M, & Bai X. (2016). MicroRNA-130b promotes lung cancer progression via PPARγ/VEGF-A/BCL-2-mediated suppression of apoptosis. Journal of Experimental & Clinical Cancer Research : CR, 35, 105.

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Transfection and Culture of U251 Cells

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The cells were grown in Dulbecco's modified Eagle's medium (DMEM; Invitrogen, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBS; Invitrogen) and 1% penicillin/streptomycin (Invitrogen/Thermo Fisher Scientific Inc., Waltham, MA, USA) in 5% CO₂ atmosphere at 37°C. The U251 cells (1×10⁵) were seeded into 6-well plates and transfected with either the negative control (NC), miR-130b mimic, miR-130b inhibitor or siPPAR-γ (5′-AAUAUGACCUGAAGCUCCAAGAAUAAG-3′) which were purchased from GenePharma (Shanghai, China), using Lipofectamine 2000 (Invitrogen) according to the manufacturer's instructions. Following a 24 h transfection, the media were removed and the cells were placed in complete medium and maintained at 37°C in an atmosphere of 5% CO₂.

GU J.J., ZHANG J.H., CHEN H.J, & WANG S.S. (2016). MicroRNA-130b promotes cell proliferation and invasion by inhibiting peroxisome proliferator-activated receptor-γ in human glioma cells. International Journal of Molecular Medicine, 37(6), 1587-1593.

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