3 4 5 dimethyl 2 thiazolyl 2 5 diphenyl 2h tetrazolium bromide mtt reagent

Hexaconazole (C₁₄H₁₇C_l2N₃O, with molecular weight of 314.21 g/mol) and dazomet (C₅H₁₀N₂S₂, with molecular weight of 162.27 g/mol) were purchased from Aiteng (Changzhou, China) with 95% and 98% purity, respectively, and were used as received. V79-4 Chinese hamster lung cell (Cricetulus griseus fibroblast, CCL-93^TM), with passage 4 and 3T3-Swiss albino ATCC ^® CCL-92™ Mus musculus embryo were bought from American Type Culture Collection (Manassas, VA, USA). Low melting points agarose (LMA) and normal melting point agarose (NMA), acetone, uranyl acetate, hydrogen peroxide, ethidium bromide solution and 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) reagent were obtained from Sigma Aldrich (St. Louis, MO, USA). Dubelcco Modified Eagle Media (DMEM), fetal bovine serum, penicillin–streptomycin, sodium cacodylate buffer solution, glutaraldehyde, and phosphate buffer saline were purchased from Gibco–Fisher Scientific (England, UK). Dimethyl sulfoxide, osmium tetroxide, and phosphate-buffered (PBS) saline were procured from Merck (Darmstadt, Germany). Propidium iodide staining solution, annexin V binding buffer and FITC-Annexin V apoptosis staining/detection kit were bought from BD Bioscience (San Jose, CA, USA). Copper grids (3 mm, 200 mesh) were acquired from Agar Scientific (Essex, UK).