Athymic BALB/c-nu/nu male nude mice and NOD/SCID male mice (GemPharmatech Co., Ltd., Jiangsu, CN), both 4–6 weeks old, were used to construct the PDOX models. The mice were maintained in the individual ventilated cages (IVC) of a specific pathogen free (SPF) facility under standard conditions with 12-h light-dark cycles and allowed free access to food and water throughout the entire experiment. All animal experiments were performed in accordance with the guidelines approved by the Institutional Animal Care and Ethics Committee of Sichuan University (approval no. 2019198A).
BxPC-3 cells (a human pancreatic adenocarcinoma cell line) were obtained from the ATCC (Virginia, USA), cultured in RPMI-1640 (GIBCO, USA) supplemented with 10% fetal bovine serum (GIBCO, USA) and 100 U/mL penicillin/streptomycin that was changed every two days and incubated at 37°C in a humidified atmosphere containing 5% CO2.
BxPC-3 cells (a human pancreatic adenocarcinoma cell line) were obtained from the ATCC (Virginia, USA), cultured in RPMI-1640 (GIBCO, USA) supplemented with 10% fetal bovine serum (GIBCO, USA) and 100 U/mL penicillin/streptomycin that was changed every two days and incubated at 37°C in a humidified atmosphere containing 5% CO2.