Luria bertani medium lb broth

All chemicals were purchased from Sigma-Aldrich
and used without further purification unless otherwise noted. Poly(ethylene
glycol) diacrylate (M_n = 250 g/mol) was purchased from Sigma-Aldrich and purified using
aluminum oxide 90 active neutral (0.063–0.200 mm, Merck KGaA).
Luria–Bertani medium (LB broth) and LB agar (Luria/Miller)
were purchased from Carl Roth. Throughout the whole study, Milli-Q
water was drawn from a Millipore Direct Q8 system (Millipore Advantage
A10 system, Schwalbach, with Millimark Express 40 filter, Merck, Germany)
with a resistivity 18.2 MΩ cm.

Alginic acid sodium salt, glycidyl methacrylate,
triethylamine, tetrabutylammonium bromide, calcium chloride
hexahydrate, sodium chloride, 2-hydroxy-4′-(2-hydroxyethoxy)-2-methylpropiophenone
(Irgacure 2959), hexamethyldisiloxane, Trizma base, Fluoresceinamine
isomer I, N-(3-(dimethylamino)propyl)-N′-ethylcarbodiimide hydrochloride (EDC), N-hydroxysulfosuccinimide sodium salt (Sulfo-NHS),
2-(N-morpholino)ethanesulfonic acid (MES) hydrate,
and N-(3-oxododecanoyl)homoserine lactone were
purchased from Sigma-Aldrich and used without further purification.
Hydrochloric acid was purchased from Fisher Chemical. N-Dodecanoyl-l-homoserine lactone-3-hydrazone fluorescein
was purchased from Biomol. Luria-Bertani medium (LB broth) and LB
agar were purchased from Carl Roth (Germany). Throughout the whole
study, Milli-Q water drawn from a Millipore Direct Q8 system with
Millimark Express 40 filter (Merck, Germany) was used.