Chicken anti-protein A polyclonal antibody (1.0 mg/mL; Arista Biological, Allentown, PA, USA; the purity of protein A > 98% at 214 nm by HPLC) and goat anti-human IgG antibody (1.0 mg/mL; Lampire Biological Laboratories, Pipersville, PA, USA) were dispensed to the nitrocellulose membrane (UniSart CN140; Sartorius Stedim Biotech SA) to be the test and the control lines, respectively, by using a lateral flow dispenser (XYZ3000 Dispensing Platform; BioDot Inc., Irvine, CA, USA; 1 µL/cm) and dried at 37°C for 1 h. The four parts of LFIA including sample pad (cytosep 1660; Pall Gelman Sciences), nitrocellulose membrane [UniSart CN140 NCM (Sartorius Stedim Biotech SA)], conjugate pad (GF33; Whatman Schleicher & Schuell), and absorbent pad (Whatman ABS. PAD #470; Whatman Schleicher & Schuell) were assembled as described previously (8 (link)). The treated glass fiber conjugate pad, sample pad, and absorbent pad were assembled to the backing card with a 0.2 cm overlap between each component (Fig. 1 ). Then the LFIA was cut into 4 mm × 6 cm strips using a CM5000 Guillotine Cutter (BioDot Inc., Irvine, CA, USA). The strips were kept at 4°C in a desiccator.
Xyz3000 dispensing platform
Manufactured by BioDot
Sourced in United States
The XYZ3000 is a dispensing platform designed for precise and consistent liquid handling. It features a multi-channel pipetting system capable of accurately dispensing a wide range of liquid volumes. The XYZ3000 is equipped with advanced automation capabilities to streamline various laboratory workflows.
Automatically generated - may contain errors
Lab products found in correlation
Cm2000 guillotine cutter, by BioDot (3 mentions)
F 4500 fluorescence spectrometer system, by Hitachi (3 mentions)
Agilent 1100 high performance liquid chromatography system, by Agilent Technologies (2 mentions)
Unisart cn140, by Sartorius (1 mentions)
Cm5000 guillotine cutter, by BioDot (1 mentions)
Milli q water purification system, by Merck Group (1 mentions)
Cm 4000, by BioDot (1 mentions)
6 protocols using xyz3000 dispensing platform
1
Lateral Flow Immunoassay Assembly Protocol
2
Automated Cutting and Dispensing for Biosensing
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The cutting module CM4000 and XYZ3000 dispensing platform were purchased from BioDot Inc. (Irvine, CA, USA). The ICA strip reader was obtained from Beijing Nanjing Biological Technology Co., Ltd (Beijing, China). A vortex mixer (CAT: HQ-60-II) was purchased from Beijing North TZ-Biotechnology Development Co. (Beijing, China), and the centrifuge (CAT: 5804R) was supplied by Eppendorf AG (Hamburg, Germany). High-purity deionized water used throughout the research was obtained using a Milli-Q water purification system (Millipore, Bedford, MA).
3
Fiberglass Conjugate Pad Preparation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Ten percent BSA was sprayed onto a fiberglass conjugate pad by using a XYZ-3000 dispensing platform (BioDot, USA). The pad was then dried at 42℃ for 45 min. Conjugate solution was dispensed onto the modified fiberglass at a speed of 25 µL/cm (about 2 µg per pad) by using the dispensing platform. After drying for 1 h at 42℃, the pad was then stored in a desiccator at room temperature.
The sample pad was saturated with a buffer containing 20 mM sodium borate, 2.0% (w/v) sucrose, 2.0% (w/v) BSA, and 0.1% (w/v) NaN3 then dried and stored as described above.
The sample pad was saturated with a buffer containing 20 mM sodium borate, 2.0% (w/v) sucrose, 2.0% (w/v) BSA, and 0.1% (w/v) NaN3 then dried and stored as described above.
4
Upconversion Nanoparticle-based Immunoassay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
An XYZ3000 dispensing platform and CM2000 guillotine cutter (BioDot, Irvine, CA, USA) were used to prepare the immunochromatographic strip. A Hitachi F-4500 fluorescence spectrometer system (Hitachi, Tokyo, Japan) was used to record the fluorescence spectrum. The UCNP-based LFIC (UCNP-LFIC) strips were scanned using a strip reader (Suzhou Helmen Precise Instruments, Suzhou, Jiangsu, China) with 980-nm-wavelength near-infrared laser excitation.
5
Fluorescent Immunochromatographic Strip Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
An XYZ3000 dispensing platform and CM2000 guillotine cutter (BioDot, Irvine, CA, USA) were used to prepare test strips. The Hitachi F-4500 fluorescence spectrometer system (Hitachi, Tokyo, Japan) was used to record the fluorescent spectrum. The FIC-S2011-B14 fluorescent strip reader (Suzhou Helmen Precise Instruments, Suzhou, Jiangsu, China) was used to scan strip results. The immunochromatographic strip results were compared with an Agilent 1100 high-performance liquid chromatography system (Agilent Tech, Santa Clara, CA, USA).
6
Fluorometric Test Strip Assay Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The role of the Hitachi F-4500 fluorescence spectrometer system (Hitachi, Tokyo, Japan) was used to read and record the fluorescence pattern. An XYZ3000 dispensing platform and a CM2000 guillotine cutter (BioDot, Irvine, CA, United States) was used to prepare and cut test strips. The purpose of the FIC-S2011-B14 fluorescent strip reader (Suzhou, Jiangsu, China) was used to read and record the results of the fluorometric test strip assay. Agilent 1,100 high performance liquid chromatography system (Agilent Tech, Santa Clara, CA, United States) was used to monitor the results of validated fluorescent test strips.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!