Polyethyleneimine pei

Manufactured by Thermo Fisher Scientific

Sourced in United States

Polyethyleneimine (PEI) is a cationic polymer commonly used in various laboratory applications. It serves as a transfection reagent, facilitating the introduction of nucleic acids, such as DNA and RNA, into cells. PEI can form complexes with these biomolecules, which can then be taken up by the cells, enabling various genetic manipulation and analysis procedures.

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Close spelling variants of this product

Polyethyleneimine (7 citations) Branched polyethyleneimine (PEI) (5 citations)

4 protocols using polyethyleneimine pei

Synthesis of Multifunctional Nanoparticles

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Ferric chloride hexahydrate (99%), ferrous chloride tetrahydrate (99%), dextran (DX) (M.w. 20,000), zinc chloride (99%), silver nitrate (99.8%), oleylamine (OLA) (70%), benzyl ether (98%), octylphenyl-polyethylene glycol (IGEPAL CA-630), poly(isobutylene-alt-maleic andydride) (PBMA) were purchased from Sigma-Aldrich. Glutathione (GSH) (98%), tetramethylammonium hydroxide (TMAH) (25% solution in methanol), iron(III) acetylacetonate (99%), myristic acid (MA) (99.5%) were purchased from Acros Organics; sodium diethyldithiocarbamate (98%), indium acetate (99.99%), polyethyleneimine (PEI) (99%, M.w. 10,000), zinc acetate (99.98%), copper(I) iodide (99.998%), tetraethylorthosilicate (TEOS) (99%), 3-mercaptopropyltrimethoxysilane (MPTMS) (97%), 1-dodecanethiol (DDT) (98%), dodecylamine (DDA) (98%) were purchased from Alfa Aesar, 3-glycidyloxypropyl-trimethoxysilane (GOPTS) (95%) was from TCI America. All chemical and organic solvents were obtained commercially as analytical-graded materials without further purification.

Koktysh D.S, & Pham W. (2019). A Combinatorial Approach for the Fabrication of Magneto-Optical Hybrid Nanoparticles. International Journal of Nanomedicine, 14, 9855-9863.

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Graphite Nanosheets Functionalization

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Graphite
powder was purchased from Sinopharm
Chemical Reagent Co., Ltd. Dimethylformamide (DMF), 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide
(EDC), and N-hydroxysuccinimide (NHS) were purchased
from Sigma-Aldrich. NH₂-PEG-NH₂ (M_w = 2000) was purchased from Jenkem Co., Ltd. Poly(ethylene
imine) (PEI, M_w = 15,000) was purchased
from Alfa Aesar. Alexa Fluor NHS ester and Goat anti mouse IgG were
purchased from Thermo Fisher Scientific. Phosphate buffered saline
(PBS, pH 7.4) was purchased from Beijing Biosynthesis Biotechnology
Co., Ltd. All the reagents were used as received. Deionized water
(Millipore Milli-Q grade) with a resistivity of 18.2 MΩ/cm was
used in all the experiments.

Lu J., Zong S., Wang Z., Chen C., Zhang Y., Wang H, & Cui Y. (2021). Dual-Labeled Graphene Quantum Dot-Based Förster Resonance Energy Transfer Nanoprobes for Single-Molecule Localization Microscopy. ACS Omega, 6(13), 8808-8815.

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Synthesis of Metal Oxide Nanoparticles

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KNO₃ (Vekton, St Petersburg, Russia, 99.9%), Bi₂O₃ (Vekton, St Petersburg, Russia, 99.9%), and TiO₂ (Vekton, St Petersburg, Russia, 99.9%) were dried at 200, 600, and 1000 °C, respectively. Methylamine (MeNH₂, 38% solution in water, Chemical line, St Petersburg, Russia,), ethylamine (EtNH₂, 70% solution in water, Merck, Darmstadt, Germany), n-propylamine (PrNH₂, Sigma-Aldrich, St Louis, MI, USA, 98%), n-butylamine (BuNH₂, 99.9%, Chemical line, St Petersburg, Russia,), n-hexylamine (HxNH₂, Sigma-Aldrich, St Louis, MI, USA, 99.9%), n-heptane (ECOS, Moscow, Russia, 99.9%), TBAOH (40 wt. % solution in water, Acros Organics, NJ, USA); polyethyleneimine (PEI, approx. M.N. 60,000, branched, 50 wt. % solution in water, Acros Organics, NJ, USA) were used as received.

Minich I.A., Silyukov O.I., Kurnosenko S.A., Gak V.V., Kalganov V.D., Kolonitskiy P.D, & Zvereva I.A. (2021). Physical–Chemical Exfoliation of n-Alkylamine Derivatives of Layered Perovskite-like Oxide H2K0.5Bi2.5Ti4O13 into Nanosheets. Nanomaterials, 11(10), 2708.

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Recombinant Protein Purification Protocol

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All chemicals and reagents used in this work were purchased from Sigma-Aldrich unless specified otherwise. GoTaq® DNA polymerase was purchased from Promega. NheI-HF®, EcoRV-HF® and T4 DNA ligase were acquired from New England Biolabs. A QIAquick PCR Purification Kit and a QIAquick Gel Extraction Kit were purchased from Qiagen. XL10-Gold® Ultracompetent cells were purchased from Agilent. E. coli BL21(DE3) Star competent cells were purchased from Themo Fisher Scientific. Anhydrotetracycline was purchased from Cayman Chemical Company. StrepTrap HP 5 mL and HisTrap HP 1 mL columns were acquired from GE Healthcare. Nicotinamide adenine dinucleotide oxidised (NAD⁺) and reduced (NADH) forms were purchased from Apollo Scientific. Relisorb® EP400SS was donated by Resindion. Polyethyleneimine (PEI) (M.N. 60 000, 50% aq. solution, branched) was purchased from Acros Organics. 2-(1H-Pyrazol-3-yl)ethanol and betazole were acquired from Fluorochem.

Romero-Fernandez M, & Paradisi F. (2021). Biocatalytic access to betazole using a one-pot multienzymatic system in continuous flow. Green Chemistry, 23(12), 4594-4603.

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