Cm10 tem

Manufactured by Olympus

The CM10 TEM is a transmission electron microscope (TEM) manufactured by Olympus. It is designed to provide high-resolution imaging and analysis of materials at the nanoscale level. The CM10 TEM allows for the observation and examination of samples by transmitting a beam of electrons through them, enabling magnification and visualization beyond the capabilities of traditional optical microscopes.

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Lab products found in correlation

Sis megaview g2 digital camera, by Olympus (2 mentions) Morada camera, by Olympus (1 mentions) Radius software, by Olympus (1 mentions) Megaview g3, by Olympus (1 mentions) Talos f200x s tem, by Thermo Fisher Scientific (1 mentions)

4 protocols using cm10 tem

Transmission Electron Microscopy Fixation

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ETR-1 and control-depleted animals were treated for 16–20 h as described above. For fixation for TEM, the conventional two-step fixation (Hall, 1995) was followed. Digital images were collected from thin sections on a Philips CM10 TEM using an Olympus Morada camera.

Boateng R., Nguyen K.C., Hall D.H., Golden A, & Allen A.K. (2017). Novel functions for the RNA-binding protein ETR-1 in Caenorhabditis elegans reproduction and engulfment of germline apoptotic cell corpses. Developmental biology, 429(1), 306-320.

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Ultrastructural Analysis of Fly Brains

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Flies were decapitated and the heads were perfused with 3% glutaraldehyde in 0.1 M sodium cacodylate buffer and 5 mM CaCl₂ overnight. Brains were then treated with 1% osmium tetroxide for 3 h at 4°C and embedded in Araldite® CY212 resin. Thin sections were stained with toluidine blue and the brain visualized with the light microscope. Ultrathin sections (70 µm) were stained with lead citrate and uranyl acetate and digital images taken on a Philips CM10 TEM with MegaView G3 digital imaging system with Radius Software (Olympus). Mitochondrial morphology was measured blind to genotype.

Manole A., Jaunmuktane Z., Hargreaves I., Ludtmann M.H., Salpietro V., Bello O.D., Pope S., Pandraud A., Horga A., Scalco R.S., Li A., Ashokkumar B., Lourenço C.M., Heales S., Horvath R., Chinnery P.F., Toro C., Singleton A.B., Jacques T.S., Abramov A.Y., Muntoni F., Hanna M.G., Reilly M.M., Revesz T., Kullmann D.M., Jepson J.E, & Houlden H. (2017). Clinical, pathological and functional characterization of riboflavin-responsive neuropathy. Brain, 140(11), 2820-2837.

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Nanocrystal Morphology Characterization

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The morphology of the formed materials was characterized via transmission electron microscopy (TEM) imaging (Philips CM10 TEM with Olympus Sis Megaview G2 Digital Camera) with an operating voltage of 100 kV. The samples were prepared by placing a drop of a dilute suspension of nanocrystals onto copper grids. High-angle annular dark-field (HAADF) scanning transmission electron microscope (STEM) images were collected with a Talos F200X S/TEM (Thermo Scientific™).

Wen S., Liu Y., Wang F., Lin G., Zhou J., Shi B., Suh Y.D, & Jin D. (2020). Nanorods with multidimensional optical information beyond the diffraction limit. Nature Communications, 11, 6047.

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Nanoparticle Morphology Characterization via TEM

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The morphology of the nanoparticles was characterised via transmission electron microscopy (TEM) imaging (Philips CM10 TEM with Olympus Sis Megaview G2 Digital Camera) with an operating voltage of 100 kV. The samples were prepared by placing a drop of a dilute suspension of nanocrystals onto copper grids.

Chen C., Liu B., Liu Y., Liao J., Shan X., Wang F, & Jin D. (2021). Heterochromatic Nonlinear Optical Responses in Upconversion Nanoparticles for Super-Resolution Nanoscopy. Advanced materials (Deerfield Beach, Fla.), 33(23).

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