Cell dyn sapphire platform

Epidemiological, demographic, clinical, and laboratory examinations were collected from all subjects at the time of admission. The data recorded included time from symptom onset to hospital admission and to CTPA, medical treatment during hospitalization, thromboprophylaxis, respiratory support, clinical outcomes (acute respiratory failure, arrhythmia, ICU admission, or death), and strong-moderate PE risk factors. The CURB-65 score was calculated (27 (link)).
Laboratory data included complete blood count (Cell-Dyn Sapphire platform, Abbott Diagnostics, United States), coagulation, and kidney and liver function tests collected upon admission. In addition, baseline, peak, and prior-to-CTPA values of the following biomarkers were analyzed in each patient: fibrinogen, D-dimer (reported as D-dimer units (DDUs), ACL TOP 700, Instrumentation Laboratory), C-reactive protein (CRP), lactate dehydrogenase (LDH), erythrocyte sedimentation rate (ESR), and ferritin. In addition, high sensitive troponin I, interleukin-6 (IL-6), interleukin-10 (IL-10) (ELISA, R&D systems), N-terminal pro hormone B-type natriuretic peptide (NT pro-BNP) (Test 1 THL Module, ALI FAX; Architect platform, Abbott Diagnostics), and fibrinogen were also measured. Blood gas analyses were performed on the GEM 4000 platform (Werfen, Spain). D-dimer-to-ferritin, D-dimer-to-LDH, and D-dimer-to-CRP ratios were calculated.

The morning after the PSG, anthropometric variables, and blood samples were collected from all women in fasting conditions. Laboratory data included complete blood count (Cell-Dyn Sapphire Platform, Abbott Diagnostics), coagulation, kidney and liver function tests (Architect c16000 platform. Abbott Diagnostics, US). Insulin was determined by processing serum samples on the Cobas e-411 platform (Roche Diagnostics GmbH, Germany) with a reference range of 3–25 μUI/mL. The Homeostatic model assessment of insulin resistance index (HOMA-IR) was calculated by the usual formula (28 (link)).
The remaining blood sample was centrifuged at 2,500 rpm for 10 min to isolate the plasma. The different plasma aliquots were stored at −80°C for further determinations.
The inflammatory cytokines were analyzed on plasma samples by multiplex technique using the Human High Sensitivity Cytokine Base Kit A (Magnetic Luminex® Performance Assay, R&D Systems®, Inc.) following the indicated procedure. For each one of the cytokines, analyzed the detection limits and coefficients of variation were 0.29 pg/mL and 5.2% (TNF-α), 0.08 pg/mL and 5.3% (IL-1β), 0.14 pg/mL and 5.2% (IL-6), 0.04 pg/mL and 6.6% (IL-8), 0.21 pg/mL and 5.4% (IL-10).
Following the delivery day, anthropometric and neonatal variables were collected from all newborns. Laboratory researchers were blinded to maternal status.