Anti human p21

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Anti-human p21 is a laboratory reagent used for the detection and quantification of the p21 protein in human samples. It is a specific antibody that binds to the p21 protein, allowing for its identification and measurement in various experimental procedures.

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Anti-p21 (199 citations) Rabbit anti-human p21 (3 citations) Human p21 (2 citations) Mouse anti‐human p21 mAb (F‐5; (2 citations)

2 protocols using anti human p21

Immunoblotting analysis of key proteins

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The following antibodies were used for immunoblotting: anti-HRAS (Santa Cruz, sc-29), anti-human p21 (Santa Cruz, sc-397), anti-E1A (Santa Cruz, sc-430); anti-ß-actin (Sigma A5441), anti-Cyclin A2 (Sigma C4710), anti-human p53 (DO-1, Sigma P6874), anti-MDM2 (clones 2A10 and 4B11) [18 (link)], anti-Histone H3 (Abcam ab1791), anti-HMGA2 (Santa Cruz, sc-30223), anti-SCD/Scd1 (Cell Signaling, #2438), anti-mouse p53 (Biovision #3036) and anti-mouse p21 (Santa Cruz #sc-6246), anti-α-Tubulin (Abcam #Ab18251), anti-SREBP1 (Santa Cruz, sc-13551). Immunoblotting analysis was carried out as described [18 (link)].

Kirschner K., Samarajiwa S.A., Cairns J.M., Menon S., Pérez-Mancera P.A., Tomimatsu K., Bermejo-Rodriguez C., Ito Y., Chandra T., Narita M., Lyons S.K., Lynch A.G., Kimura H., Ohbayashi T., Tavaré S, & Narita M. (2015). Phenotype Specific Analyses Reveal Distinct Regulatory Mechanism for Chronically Activated p53. PLoS Genetics, 11(3), e1005053.

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Protein Expression Analysis in Cells

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Total protein from cells were lyzed by RIPA buffer. The GAPDH was regarded as the endogenous normalizer. The polyclonal rabbit anti-human Cdk2 (sc-748), Cyciln A (sc-596), Cyciln D1 (sc-717) (Santa Cruz, USA) and mouse monoclonal anti-human p21 (sc-817), p53 (sc-47698), Bcl-2 (sc-509), Bcl-xL (sc-136132), Bax (sc-6236), caspase3 (sc-136219), caspase7 (sc-81654), caspase9 (sc-56073), E-cadherin (sc-71009), snail (sc-393172), Vimentin (sc-73259), survivin (sc-101433), STAT3 (sc-293151), p-STAT3 (sc-8001-R), PARP (sc-136208) (Santa Cruz, USA) and GAPDH (SRP00849) (Saierbio, China) antibody were used.

Zhao X., Huang L., Xu W., Chen X., Shen Y., Zeng W, & Chen X. (2017). Physapubescin B inhibits tumorgenesis and circumvents taxol resistance of ovarian cancer cells through STAT3 signaling. Oncotarget, 8(41), 70130-70141.

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