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Pe cy7 tcr

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The PE/Cy7-TCR is a fluorescently-labeled antibody used to detect and quantify T cell receptor (TCR) expression on the surface of T cells. It is a dual-labeled conjugate, combining the Phycoerythrin (PE) and Cyanine 7 (Cy7) fluorescent dyes. This combination allows for multicolor flow cytometric analysis of TCR expression alongside other cell surface markers.

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Spelling variants of this product

Anti-TCR α/β-PE/Cy7 (3 citations) PE/Cy7 anti-TCR β chain (1 citations) Anti-mouse TCR β chain PE/Cy7 (1 citations)

2 protocols using pe cy7 tcr

1

Flow Cytometric Analysis of CAR-T Cell Phenotype

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Surface protein expression was determined by flow cytometry. After electroporation for 5 days, 1×106 cells were incubated with APC-CD4, PE/Cy7-TCR (or PE-TCR) and FITC-CD3 antibodies (Biolegend) for 30 mins. For the CD22BBz CAR transduced T cells were incubated with 0.2 μg CD22-Fc (R&D system) in 100 μL PBS for 30 mins, and then stained with PE-IgG-Fc (Biolegend). For the CD19BBz CAR detection, the transduced T cells were stained with APC-anti-DYKDDDDK Tag (Biolegend). Stained cells were measured and sorted on BD FACSAria II. For the T cell exhaustion assay, T cells from various groups were co-cultured with NALM6 cells at 0.5:1 E:T ratio for 24 hours. 1×106 cells were incubated with 0.2 μg CD22-Fc (R&D Systems) in 100 μL PBS for 30 mins and then stained with PE-IgG-Fc, PD-1-FITC, TIGIT-APC and LAG3-Percp/cy5.5 (Biolegend) for 30 mins. After washing twice, the stained cells were measured and sorted on BD FACSAria II, and analyzed using FlowJo software version 9.9.4 or 10.3 (Treestar, Ashland, OR).
Dai X., Park J.J., Du Y., Kim H.R., Wang G., Errami Y, & Chen S. (2019). One-step generation of modular CAR-T with AAV-Cpf1. Nature methods, 16(3), 247-254.
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2

Flow Cytometric Analysis of CAR-T Cell Phenotype

Check if the same lab product or an alternative is used in the 5 most similar protocols
Surface protein expression was determined by flow cytometry. After electroporation for 5 days, 1×106 cells were incubated with APC-CD4, PE/Cy7-TCR (or PE-TCR) and FITC-CD3 antibodies (Biolegend) for 30 mins. For the CD22BBz CAR transduced T cells were incubated with 0.2 μg CD22-Fc (R&D system) in 100 μL PBS for 30 mins, and then stained with PE-IgG-Fc (Biolegend). For the CD19BBz CAR detection, the transduced T cells were stained with APC-anti-DYKDDDDK Tag (Biolegend). Stained cells were measured and sorted on BD FACSAria II. For the T cell exhaustion assay, T cells from various groups were co-cultured with NALM6 cells at 0.5:1 E:T ratio for 24 hours. 1×106 cells were incubated with 0.2 μg CD22-Fc (R&D Systems) in 100 μL PBS for 30 mins and then stained with PE-IgG-Fc, PD-1-FITC, TIGIT-APC and LAG3-Percp/cy5.5 (Biolegend) for 30 mins. After washing twice, the stained cells were measured and sorted on BD FACSAria II, and analyzed using FlowJo software version 9.9.4 or 10.3 (Treestar, Ashland, OR).
Dai X., Park J.J., Du Y., Kim H.R., Wang G., Errami Y, & Chen S. (2019). One-step generation of modular CAR-T with AAV-Cpf1. Nature methods, 16(3), 247-254.
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