Protein samples were mixed with 4× SDS loading buffer. Samples were boiled for 5 minutes at 98°C, and proteins were separated by Biofuraw Precast Gel (Tanon) and transferred to PVDF membranes. The following primary Abs and dilutions were used: Snail2 mAb (1:100, c‐166467; Santa Cruz Biotechnology), E‐cadherin polyclonal Ab, vimentin polyclonal Ab, fibronectin polyclonal Ab, and GAPDH polyclonal Ab (1:1000, 874‐1‐AP, 15613‐1‐AP, 10366‐1‐AP, 60004‐1‐AP; Proteintech). Secondary Abs were anti‐rabbit or anti‐mouse HRP‐conjugated IgG (1:2000, SA00001‐4; Proteintech). Membranes were incubated with 1 mL ECL western blotting substrate (Promega) for 1 minute at room temperature and then exposed to X‐ray film.
C 166467
Manufactured by Santa Cruz Biotechnology
C‐166467 is a laboratory equipment product offered by Santa Cruz Biotechnology. It is a device designed for use in research and scientific applications. The core function of this product is to perform a specific technical task, without further interpretation or extrapolation on its intended use.
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Lab products found in correlation
2 protocols using c 166467
1
Western Blot Analysis of EMT Markers
2
Western Blot Analysis of EMT Markers
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Protein samples were mixed with 4× SDS loading buffer. Samples were boiled for 15 min at 98°C, and proteins were separated by Biofuraw™ Precast Gel (Tanon) and transferred to NC membranes, and then, the following primary antibodies and dilutions were used: Snail2 monoclonal antibody (1:1000, c-166,467, Santa Cruz,), E-cadherin polyclonal antibody, vimentin polyclonal antibody, fibronectin polyclonal antibody, and GAPDH polyclonal antibody (1:1000, 874–1-AP, 15,613–1-AP, 10,366–1-AP, 60,004–1-AP, Proteintech). Secondary antibodies were anti-rabbit or anti-mouse HRP-conjugated IgG (1:2000, SA00001-4, Proteintech). Membranes were incubated with 1 mL ECL western blotting substrate (Promega) for 1 min at room temperature and then exposed to x-ray film.
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