R-amlodipine and S-amlodipine were purchased from Santa Cruz Biotechnology Inc. (Heidelberg, Germany). Ethoxyresorufin, 7-ethoxy-4-(trifluoromethyl)Coumarin and 4′-hydroxydiclofenac were purchased from Fluka (Buchs, Switzerland). Coumarin, testosterone, diclofenac, bufuralol, chlorzoxazone, resorufin, 7-hydroxyCoumarin, 7-hydroxy-4-(trifluoromethyl)Coumarin, 1′-hydroxymidazolam and 1′-hydroxybufuralol were obtained from Sigma-Aldrich (Prague, Czech Republic). Midazolam was purchased from Abcam (Cambridge, UK), 6β-hydroxytestosterone was purchased from Ultrafine (Manchester, UK), and paclitaxel from Chemos CZ (Prague, Czech Republic). The 6-hydroxypaclitaxel and S-mephenytoin were purchased from Santa Cruz Biotechnology (Heidelberg, Germany), and (S)-4-hydroxy mephenytoin was bought from Toronto Research Chemicals Inc. (Toronto, Canada). Human liver microsomes and genotyped human liver microsomes (Table 5 ) were obtained from XenoTech (Lenexa, KS, USA). The activities of the CYP1A, CYP2A6, CYP2B6, CYP2C8, CYP2C9, CYP2C19, CYP2D6, CYP2E1, and CYP3A enzymes can be accessed from the XenoTech web site (www.xenotechllc.com ).
4 hydroxy s mephenytoin
Manufactured by LGC
Sourced in Canada
4-hydroxy-S-mephenytoin is a chemical compound used in research and laboratory applications. It serves as a reference standard and analytical tool for the identification and quantification of this specific compound in various samples and applications. The core function of 4-hydroxy-S-mephenytoin is to provide a reliable and well-characterized reference material for analytical purposes.
Automatically generated - may contain errors
Lab products found in correlation
Coumarin, by Merck Group (4 mentions)
Chlorzoxazone, by Merck Group (3 mentions)
6β hydroxytestosterone, by Merck Group (3 mentions)
S mephenytoin, by Cayman Chemical (3 mentions)
Testosterone, by Cayman Chemical (3 mentions)
Diclofenac sodium salt, by Cayman Chemical (3 mentions)
4 hydroxydiclofenac, by Cayman Chemical (3 mentions)
7 hydroxycoumarin, by Chem Service (3 mentions)
Testosterone, by Merck Group (2 mentions)
Resorufin, by Merck Group (2 mentions)
Acetaminophen, by Merck Group (2 mentions)
Phenacetin, by Merck Group (2 mentions)
4 aminobenzoic acid, by Merck Group (2 mentions)
Dextromethorphan hydrobromide, by Merck Group (2 mentions)
Paclitaxel, by Cayman Chemical (2 mentions)
Benzydamine hydrochloride, by Merck Group (2 mentions)
Kynuramine hydrobromide, by Santa Cruz Biotechnology (2 mentions)
6 hydroxychlorzoxazone, by LGC (2 mentions)
1 hydroxymidazolam, by LGC (2 mentions)
7 hydroxycoumarin β d glucuronide sodium salt, by Merck Group (2 mentions)
5 protocols using 4 hydroxy s mephenytoin
1
In Vitro CYP Enzyme Assays
2
Quantitative Bioanalysis of Xenobiotic Metabolites
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Progesterone (P), 17α-hydroxyprogesterone (17α-OHP), estrone (E1), estradiol (E2), estriol (E3), human growth hormone (hGH), bovine serum albumin (BSA), phenacetin, S-mephenytoin, dextromethorphan hydrobromide monohydrate, testosterone, 6β-hydroxy testosterone, acetaminophen, and all solvents of the highest purity LC–MS/MS grade were obtained from Sigma-Aldrich Inc. (St. Louis, MO). Diclofenac sodium salt and dextrorphan-d-tartrate were purchased from MP Biomedical Inc. (Solon, OH, USA). 4-Hydroxy diclofenac, (S)-4-hydroxy mephenytoin, and deuterated internal standards for the respective metabolites acetaminophen-D4, 4-Hydroxy diclofenac-D4, ( ±)-4-hydroxy mephenytoin-D3, dextrorphan-D3 tartrate salt, and 6β-hydroxy testosterone-D3 were procured from Toronto Research Chemicals Inc. (Ontario, Canada). Cell lysis buffer was purchased from Roche Diagnostics Corporation (Indianapolis, IN). Rabbit anti-human primary monoclonal antibodies against CYP3A4 and β-actin and anti-rabbit IgG linked with horseradish peroxidase were obtained from Cell Signaling Technology, Inc. (Danvers, MA). Polyvinylidene difluoride (PVDF) membrane was obtained from Bio-Rad (Hercules, CA). Enhanced chemiluminescence substrate was purchased from Thermo Fisher Scientific (Rockford, IL).
3
High-Throughput Cytochrome P450 Profiling
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Acetaminophen, astemizole, bupropion, N‐acetyl‐p‐aminobenzoic acid, 4‐aminobenzoic acid, benzydamine hydrochloride, chlorzoxazone, coumarin, dextromethorphan hydrobromide, 7‐Ethoxyresorufin, 7‐Hydroxycoumarin β‐D‐glucuronide sodium salt, 7‐Hydroxycoumarin sulfate potassium salt, 6β‐hydroxy testosterone, irinotecan, midazolam, phenacetin, resorufin, SN38, and sulfamethazine were purchased from Sigma Aldrich. Dextrorphan tartrate, diclofenac sodium salt, 4‐hydroxydiclofenac, hydroxy bupropion, S‐mephenytoin, 4‐hydroxyquinoline, paclitaxel, and testosterone were purchased from Cayman Chemical. 7‐hydroxycoumarin was purchased from Chem Service. Benzydamine N‐oxide, kynuramine hydrobromide, and N‐acetyl sulfamethazine were obtained from Santa Cruz Biotechnology. Carbazeran, 4‐hydroxyCarbazeran, 6‐hydroxychlorzoxazone, 6α‐hydroxy paclitaxel, 1′‐hydroxymidazolam, and 4‐hydroxy‐S‐mephenytoin were obtained from Toronto Research Chemicals.
4
Metabolism Assay for Pharmacological Compounds
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Astemizole, irinotecan hydrochloride, diclofenac sodium salt, 7-ethyl-10-hydroxycamptothecin (SN38), 4-hydroxydiclofenac, s-mephenytoin, and testosterone were purchased from Cayman Chemical (Ann Arbor, MI). 7-HydroxyCoumarin was purchased from Chem Service (West Chester, Pennsylvania). 7-HydroxyCoumarin sulfate potassium salt was obtained from Santa Cruz Biotechnology (Dallas, Texas). Coumarin, 6β-hydroxytestosterone, 7-HydroxyCoumarin β-D-glucuronide sodium salt, (2S, 3S)-hydroxy were purchased from Sigma-Aldrich (St. Louis, MO). Midazolam, 1'-hydroxyMidazolam, O-desmethyl astemizole, and 4-hydroxy-s-mephenytoin were obtained from Toronto Research Chemicals (Toronto, Canada).
5
Acquisition of Enzyme Substrates
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Dextrorphan tartrate, diclofenac sodium salt, 4-hydroxydiclofenac, S-mephenytoin, 4-hydroxyquinoline, paclitaxel, and testosterone were purchased from Cayman Chemical (Ann Arbor, MI). 7-Hydroxycoumarin was purchased from Chem Service (West Chester, PA). Benzydamine N-oxide, 7-Hydroxycoumarin sulfate potassium salt, kynuramine hydrobromide, and N-acetyl sulfamethazine were obtained from Santa Cruz Biotechnology (Dallas, TX). 4-Acetamidobenzoic acid, p-acetamidophenyl b-D-glucuronide sodium salt, 4-aminobenzoic acid, benzydamine hydrochloride, chlorzoxazone, coumarin, dextromethorphan hydrobromide, 6b-hydroxytestosterone, 7-Hydroxycoumarin b-D-glucuronide sodium salt, 7-ethoxycoumarin, paracetamol sulfate potassium, phenacetin, and sulfamethazine were purchased from Sigma-Aldrich (St. Louis, MO). Carbazeran, 4-hydroxyCarbazeran, 6-hydroxychlorzoxazone, 6a-hydroxypaclitaxel, acetaminophen glutathione disodium salt, midazolam, 19-hydroxymidazolam, and 4-hydroxy-S-mephenytoin were obtained from Toronto Research Chemicals (Toronto, ON, Canada). All other drug-metabolizing enzyme substrates were obtained from Sigma-Aldrich.
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