CD4+CD45RA+ naive T cells were stimulated for 72 h. 5 × 104 T cells were seeded on top of a 3D collagen matrix (1.5 mg/mL, bovine collagen type I, Advanced Biomatrix; approximately 5 mm in thickness). After 48 h, collagen matrix was fixed with 4% PFA and stained with DAPI for 1 h. Collagen matrix were imaged with an LSM710 microscope with an EC Plan-Neofluar 10 × /0.3-NA (numerical aperture) DICI objective lens (Carl Zeiss). Z stacks were set to capture images from the surface to the bottom of the 3D collagen matrix.
Ec plan neofluar 10 0.3 na
Manufactured by Zeiss
The EC Plan-Neofluar 10 × /0.3-NA is a microscope objective lens produced by Zeiss. It has a magnification of 10× and a numerical aperture of 0.3. The lens is designed for use in brightfield microscopy applications.
Automatically generated - may contain errors
Lab products found in correlation
Bovine collagen type 1, by Advanced BioMatrix (1 mentions)
Bond polymer refine detection kit, by Leica (1 mentions)
Horseradish peroxidase labeled rabbit anti mouse, by Abcam (1 mentions)
Axiovision software, by Zeiss (1 mentions)
Axiolab, by Zeiss (1 mentions)
Bromodeoxyuridine (brdu), by Roche (1 mentions)
Anti ki67, by BD (1 mentions)
2 protocols using ec plan neofluar 10 0.3 na
1
3D Collagen Matrix Imaging of Activated T Cells
2
Hepatocyte Proliferation Quantification
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For detection of hepatocyte proliferation, mice were given a single, ip, injection of 100 µg/g BrdU (Roche Applied Science) 2 hours before sacrifice. Liver samples were fixed in 4% of paraformaldehyde followed by dehydration and embedded in paraffin. Tissue sections were incubated with BrdU-specific antibodies (Abcam) or anti-Ki-67 (BD Pharmingen). For visualization, horseradish peroxidaselabeled rabbit anti-mouse (Abcam) was used and detection was performed using the Bond Polymer Refine Detection Kit (DS9800) on a Bond Max staining robot (both from Leica). Individual specimens were stained with hematoxylin and eosin according to standard protocols. Stained sections were analyzed using an Axiolab attached to an AxioCamMRc5 5MPx camera, an CP-Achromat 5×/0.12 NA, an EC-Plan-Neofluar 10×/0.3 NA, and an Achroplan 20×/0.45 NA objective (all Zeiss Microscopy). For quantification, five random high-power fields for each animal were counted and the fraction of stained hepatocyte nuclei was calculated using the AxioVision Software (Zeiss Microscopy).
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