Log In Sign up
The largest database of trusted experimental protocols

Borosilicate glass beads

Manufactured by Next Advance
Visit Supplier

Borosilicate Glass Beads are laboratory equipment made of a durable, heat-resistant glass composition. They are used for various applications in scientific research and experimentation.

Automatically generated - may contain errors

Lab products found in correlation

Infinite f500, by Tecan (2 mentions) Dc protein assay, by Bio-Rad (2 mentions)

Spelling variants of this product

Glass beads (5 citations)

2 protocols using borosilicate glass beads

1

Proteome Fractionation and Quantification of C. elegans

Check if the same lab product or an alternative is used in the 5 most similar protocols
Approximately 3,000 N2 C. elegans were grown in liquid culture with irradiated Op50 (6 mg/mL) per replicate. On day 1 of adulthood, animals were treated with compound or DMSO, respectively, for 24 h, washed 6X in PBS and flash frozen until homogenization. To harvest proteomes, a small scoop each of 0.5 mm Borosilicate Glass Beads (Next Advance) and 1.4 mm Zirconium Oxide Beads (Precellys) were added to the animals and animals were homogenized in the Bullet Blender (5 min, setting 9, 2X). Cell lysates were then centrifuged (100,000 × g, 45 min) to yield membrane (pellet) and soluble (supernatant) fractions. Membrane pellets were resuspended in PBS by sonication. Protein concentrations were determined using the DC Protein Assay (Bio-Rad) and absorbance read at 750 nm using an Infinite F500 plate reader (Tecan). Proteomes were prepared at 1 mg/mL in PBS.
Chen A.L., Lum K.M., Gonzalez P.L., Ogasawara D., Cognetta AB I.I.I., To A., Parsons W.H., Simon G.M., Desai A., Petrascheck M., Bar-Peled L, & Cravatt B.F. (2019). Pharmacological convergence reveals a lipid pathway that regulates C. elegans lifespan. Nature chemical biology, 15(5), 453-462.
+ Open protocol
+ Expand
2

Proteome Fractionation and Quantification of C. elegans

Check if the same lab product or an alternative is used in the 5 most similar protocols
Approximately 3,000 N2 C. elegans were grown in liquid culture with irradiated Op50 (6 mg/mL) per replicate. On day 1 of adulthood, animals were treated with compound or DMSO, respectively, for 24 h, washed 6X in PBS and flash frozen until homogenization. To harvest proteomes, a small scoop each of 0.5 mm Borosilicate Glass Beads (Next Advance) and 1.4 mm Zirconium Oxide Beads (Precellys) were added to the animals and animals were homogenized in the Bullet Blender (5 min, setting 9, 2X). Cell lysates were then centrifuged (100,000 × g, 45 min) to yield membrane (pellet) and soluble (supernatant) fractions. Membrane pellets were resuspended in PBS by sonication. Protein concentrations were determined using the DC Protein Assay (Bio-Rad) and absorbance read at 750 nm using an Infinite F500 plate reader (Tecan). Proteomes were prepared at 1 mg/mL in PBS.
Chen A.L., Lum K.M., Gonzalez P.L., Ogasawara D., Cognetta AB I.I.I., To A., Parsons W.H., Simon G.M., Desai A., Petrascheck M., Bar-Peled L, & Cravatt B.F. (2019). Pharmacological convergence reveals a lipid pathway that regulates C. elegans lifespan. Nature chemical biology, 15(5), 453-462.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!