Illustra dna and gel band purification kit

Manufactured by GE Healthcare

Sourced in United Kingdom

The Illustra DNA and Gel Band Purification Kit is a laboratory equipment product designed for the purification of DNA fragments from agarose gels or PCR reactions. The kit provides the necessary reagents and protocols to efficiently extract and purify DNA samples, removing unwanted contaminants and impurities.

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Lab products found in correlation

Abi prism 3700 dna analyzer, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Illustra GFX PCR DNA and Gel Band Purification Kit (107 citations) Gel Band Purification Kit (36 citations) IllustraTM GFXTM PCR DNA and Gel Band Purification Kit (17 citations) Illustra GFX DNA and Gel Band Purification Kit (3 citations) Gel purification kit (2 citations)

2 protocols using illustra dna and gel band purification kit

Molecular Characterization of Drug Resistance in Falciparum Malaria

Cited 1 time

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Samples that were diagnosed as P. falciparum were further subjected to series of PCR amplification reactions targeting different genes related with drug resistance in P. falciparum (pfdhfr, pfdhps, pfmdr1, pfcrt, pfcytb, pfketch13) according to their authors [21 (link)–24 (link)].
Amplified gene products were subsequently subjected to sequencing to assess the occurrence of significant mutations. The amplified products were purified using Illustra DNA and gel band purification kit (General Electric Healthcare) and sequenced with the Big Dye Terminator v3.1 cycle sequencing in an ABI PRISM^®3700 DNA analyzer. All amplified products were sequenced in both directions twice.

Soliman R.H., Garcia-Aranda P., Elzagawy S.M., Hussein B.E., Mayah W.W., Martin Ramirez A., Ta-Tang T.H, & Rubio J.M. (2018). Imported and autochthonous malaria in West Saudi Arabia: results from a reference hospital. Malaria Journal, 17, 286.

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Skin Snip DNA Extraction and Filarial PCR

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Preparation of the DNA template: skin snip biopsies DNA were extracted using the QIAamp DNA Mini Kit with a incubation at 56°C overnight previous to DNA extraction. DNA, was eluted with 200 μl distilled water and stored at 4°C until use.
Detection of filarial species was performed by Real Time PCR, from a modification of a Nested Filaria PCR [20 (link)]. The amplified products were purified for sequencing using Illustra DNA and Gel Band Purification Kit (General Electric Healthcare, England) and sequenced using Big Dye Terminator v3.1 Cycle Sequencing in an ABI PRISM 3700 DNA Analyzer (Applied Biosystems, U.S.A.).

Moya L., Herrador Z., Ta-Tang T.H., Rubio J.M., Perteguer M.J., Hernandez-González A., García B., Nguema R., Nguema J., Ncogo P., Garate T., Benito A., Sima A, & Aparicio P. (2016). Evidence for Suppression of Onchocerciasis Transmission in Bioko Island, Equatorial Guinea. PLoS Neglected Tropical Diseases, 10(7), e0004829.

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