Cytokine and chemokine levels were determined in undiluted BAL fluid supernatant using the V-PLEX® Proinflammatory and Cytokine Panel 1 (mouse) Kit MSD® Multi-Spot Assay System (Meso Scale Diagnostics, LLC), according to protocol. Absorbance was measured on the Meso Scale Discovery (MSD) plate reader (Meso Scale Diagnostics, Maryland, USA). The following cytokines and chemokines were included in the panel: IL-4, IL-10, IFN-γ, IL-2, IL-5, KC/GRO, IL-1β, IL-12p70, TNF-α, IL-6, IL-15, IP-10, MCP-1, MIP-1α, IL-9, IL-17 A/F, IL-33, IL-27p28/IL-30 and MIP-2. Detection limits can be found in Additional File 4.
Meso scale discovery plate reader
Manufactured by Mesoscale
Sourced in United States
The Meso Scale Discovery (MSD) plate reader is a laboratory instrument designed for sensitive and quantitative measurement of analytes in biological samples. It utilizes electrochemiluminescence technology to detect and quantify a wide range of targets, including proteins, peptides, and small molecules. The MSD plate reader provides accurate and reproducible results with a high degree of sensitivity and dynamic range.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using meso scale discovery plate reader
1
Comprehensive Cytokine and Chemokine Profiling
2
Multiplex Cytokine Profiling in BAL
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cytokine and chemokine levels were determined in undiluted BAL fluid supernatant using the V-PLEX® Proinflammatory and Cytokine Panel 1 (mouse) Kit MSD® Multi-Spot Assay System (Meso Scale Diagnostics, LLC), according to protocol. Absorbance was measured on the Meso Scale Discovery (MSD) plate reader (Meso Scale Diagnostics, Maryland, USA). The following cytokines and chemokines were included in the panel: IL-4, IL-10, IFN-γ, IL-2, IL-5, KC/GRO, IL-1β, IL-12p70, TNF-α, IL-6, IL-15, IP-10, MCP-1, MIP-1α, IL-9, IL-17A/F, IL-33, IL-27p28/IL-30 and MIP-2. Detection limits can be found in Additional File 4.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!