Rudolph autopo 5

Manufactured by Rudolph Research Analytical

The Rudolph Autopo V is an automated polarimeter designed for the measurement of optical rotation. It provides accurate and reliable measurements of the optical activity of liquids and solutions.

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Lab products found in correlation

Agilent 1260 series, by Agilent Technologies (3 mentions) Agilent 1260 series dad detector, by Agilent Technologies (3 mentions) Brighttime chirascan, by Applied Photophysics (3 mentions) Agilent 6520 q tof mass spectrometer, by Agilent Technologies (2 mentions) Nicolet 6700, by Thermo Fisher Scientific (2 mentions) Avance 3 500 spectrometer, by Bruker (2 mentions) Avance 3 500, by Bruker (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Methyl thiazolyl tetrazolium (mtt), by Merck Group (1 mentions) Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions) Thermo scientific nicolet 6700, by Thermo Fisher Scientific (1 mentions) Avance 3 600, by Bruker (1 mentions)

3 protocols using rudolph autopo 5

Isolation and Purification of Natural Products

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Column chromatography (CC): silica gel H (10–40 μm) and silica gel (200–300 mesh) (Marine Chemical Factory, Qingdao, P. R. China); MCI gel CHP–20P: (Daiso, Co., Japan) and RP-C₁₈ gel (40–63 μm; Daiso, Co., Japan) were used for column chromatography; Sephadex LH-20 (Pharmacia Fine Chemicals, Piscataway, NJ, USA). TLC: silica gel plates, visualization by spraying with 10% H₂SO₄ in EtOH and Dragendorff's reagent. Semi-preparative HPLC: Agilent 1260 series (Agilent Technologies, US) with a Zorbax SB-C₁₈ (5 μM, 9.4 mm × 25 cm) column. NMR Spectra: Bruker Avance III-500 spectrometer (Bruker, Switzerland). MS: Agilent MSD-Trap-XCT (for ESI) and Agilent-6520 Q-TOF mass spectrometer (for HR-ESI). IR: Thermo Scientific Nicolet 6700 (Thermo Scientific, US). UV spectra: Agilent 1260 series DAD detector (Agilent Technologies, US). CD spectrum: Brighttime Chirascan (Applied Photophysics Ltd, UK). Optical rotation: Rudolph Autopo V (Rudolph Research Analytical, Hackettstown, NJ).

Wu Z.L., Sun Z.S., Li J.Y., Yang Y.X., Zu X.P., Li H.L, & Zhang W.D. (2022). Gerberdriasins A–F, six undescribed coumarin derivatives from Gerbera anandria (Linn) Sch-Bip and their protective effects on scopolamine-induced injury in PC12 cells. RSC Advances, 12(32), 20771-20777.

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Column Chromatography and Spectroscopic Analysis

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Column chromatography (CC): silica gel H (10–40 μm) and silica gel (200–300 mesh) (Marine Chemical Factory, Qingdao, P. R. China). MCI gel CHP-20P: (Daiso, Co., Japan) and RP-C18 gel (40–63 μm; Daiso, Co., Japan) were used for column chromatography. Sephadex LH-20 (Pharmacia Fine Chemicals, Piscataway, NJ, USA); TLC: silica gel plates, visualization by spraying with 10% H₂SO₄ in EtOH and Dragendorff's reagent. Semi-preparative HPLC: Agilent 1260 series (Agilent Technologies, US) with a Zorbax SB-C₁₈ (5 μM, 9.4 mm × 25 cm) column. NMR spectra: Bruker Avance III-500 spectrometer (Bruker, Switzerland). MS: Agilent MSD-Trap-XCT (for ESI) and Agilent-6520 Q-TOF mass spectrometer (for HR-ESI). IR: Thermo Scientific Nicolet 6700 (Thermo Scientific, US). UV spectra: Agilent 1260 series DAD detector (Agilent Technologies, US). CD spectrum: Brighttime Chirascan (Applied Photophysics Ltd, UK). Optical rotation: Rudolph Autopo V (Rudolph Research Analytical, Hackettstown, NJ).

Wu Z.L., Li J.Y., Huang P.L., Sun Z.S., Li H.L, & Zhang W.D. (2022). New ent-kaurane diterpenoid acids from Nouelia insignis Franch and their anti-inflammatory activity. RSC Advances, 12(18), 11155-11163.

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Chromatographic Purification and Analytical Methods

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Column chromatography (CC): silica gel H (10–40 μm) and silica gel (200–300 mesh) (Marine Chemical Factory, Qingdao, P. R. China); Sephadex LH-20 (Pharmacia Fine Chemicals, Piscataway, NJ, USA); RP-C18 gel (40−63 μm; Daiso, Co., Japan). TLC: silica gel plates (Yantai Jiang You Silicone Development Co., Yantai, P. R. China), visualization by spraying with 10% H₂SO₄ in EtOH. HPLC: Agilent 1260 series (Agilent Technologies, US) with a Zorbax SB-C18 (5 μm, 9.4 × 150 mm) column. NMR: Bruker Avance III-500 and Avance III-600 spectrometers (Bruker, Switzerland). MS: Agilent MSD-Trap-XCT (for ESI) and Agilent-6520 Q-TOF mass spectrometers (for HR-ESI). Melting point: X-4B digital display micro-melting apparatus (Shanghai Jingsong Instrument, Shanghai, P. R. China). Optical rotation: Rudolph Autopo V (Rudolph Research Analytical, Hackettstown, NJ). UV: Agilent 1260 series DAD detector (Agilent Technologies, US). CD: Brighttime Chirascan (Applied Photophysics Ltd, UK). IR: MTTiCZIBPBHhf-iF1qfc/" target="_blank">Thermo Scientific Nicolet 6700 (Thermo Scientific, USA). RAW 264.7 cells and 293T cells: ATCC (American type culture collection). Dulbecco’s modified Eagle’s medium and fetal bovine serum: Gibco Invitrogen (Carlsbad, CA, USA). LPS Griess reagent and MTT: Sigma-Aldrich (St. Louis, MO, USA).

Chen L.P., Wu G.Z., Zhang J.P., Ye J., Liu Q.X., Shen Y.H., Li H.L, & Zhang W.D. (2017). Vlasouliolides A-D, four rare C17/C15 sesquiterpene lactone dimers with potential anti-inflammatory activity from Vladimiria souliei. Scientific Reports, 7, 43837.

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