Retinal sections, optic nerve sections, and whole mounted retinas were analyzed under a confocal microscope (Zeiss Z1 - LSM 780, Zeiss microscopes) equipped with a computer-driven motorized stage controlled by its own image analysis software (Zen Lite Back edition 2012, Zeiss). The retinal and optic nerve photomontages were reconstructed using the maximum projection of individual images (20x) by zig-zag tiling with an overlap of 8% between frames. To characterize the GCL composition, images of retinal sections (20x) were acquired at three different retinal areas (periphery, medial and central or visual streak). However, to uncover the whole retina surface, magnifications from flat mounts retinas (63x) were acquired in five regions along the DV axis (dorsal periphery, above ONH, visual streak, medial retina and ventral periphery; 4 images/region) using the same settings.
Zen lite back edition 2012
Manufactured by Zeiss
Zen Lite Back edition 2012 is a microscopy software platform developed by Zeiss. It provides a user interface for controlling and configuring various Zeiss microscope systems. The software offers basic image acquisition and processing capabilities.
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Lab products found in correlation
2 protocols using zen lite back edition 2012
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Comprehensive Retinal Imaging and Analysis
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Comprehensive Retinal Imaging and Analysis
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Retinal sections, optic nerve sections, and whole mounted retinas were analyzed under a confocal microscope (Zeiss Z1 - LSM 780, Zeiss microscopes) equipped with a computer-driven motorized stage controlled by its own image analysis software (Zen Lite Back edition 2012, Zeiss). The retinal and optic nerve photomontages were reconstructed using the maximum projection of individual images (20x) by zig-zag tiling with an overlap of 8% between frames. To characterize the GCL composition, images of retinal sections (20x) were acquired at three different retinal areas (periphery, medial and central or visual streak). However, to uncover the whole retina surface, magnifications from flat mounts retinas (63x) were acquired in five regions along the DV axis (dorsal periphery, above ONH, visual streak, medial retina and ventral periphery; 4 images/region) using the same settings.
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