Oxaliplatin l ohp

The following antibodies were used in western blotting assays: anti-COX-2, anti-AKT, anti-ERK, anti-CDK2, anti-Ac-lysine, and anti-SIRT3 (Cell Signaling Technology, Danvers, MA, USA), anti-Drp1 and anti-p-Drp1^Ser616 (Ruiying Biological, Jiangsu, China), anti-p38 MAPK, anti-PKCα, anti-PINK1, anti-GAPDH, anti-Bax, anti-Bcl-2, anti-cleaved-Caspase-3, anti-COXIV, anti-Flag, Alexa Fluor^® 488-labeled goat anti-rabbit IgG, Alexa Fluor^® 488-labeled goat anti-mouse IgG, and DyLight^® 405-labeled goat anti-mouse IgG (Beyotime, Shanghai, China). The peroxidase-conjugated affinipure secondary antibodies goat anti-rabbit IgG and anti-mouse IgG were purchased from Thermo Fisher Scientific (Waltham, MA, USA). cDDP, RSV, dimethyl sulfoxide (DMSO), and Mdivi-1 were purchased from Sigma Aldrich (St. Louis, MO, USA). Carboplatin (CBP) was purchased from Hansoh Pharmaceutical Co., Ltd. (Jiangsu, China), and oxaliplatin (L-OHP) was purchased from Selleck (Santa Clara, TX, USA).

Oxaliplatin (L-OHP) and vincristine (VCR) were purchased from Selleck Chemicals (Houston, TX, USA). Fetal bovine serum (FBS), RPMI-1640 culture medium, penicillin, and streptomycin were purchased from Gibco (Carlsbad, CA, USA). shFOXM1 (target sequence, 5'- CTCTTCTCCCTCAGATATA-3'), shDVL2 (target sequence, 5'-GGAAGAAATTTCAGATGAC-3'), shSnail (target sequence, 5'- GCCTTCAACTGCAAATACT-3'), and shRNA negative control (shNC) were gained from Genechem (Shanghai, China). cDNAs encoding FOXM1 or Snail was respectively cloned into pcDNA3.1 or pcDNA3.1-HA. pcDNA3.1-Flag-DVL2 was derived from pCMV5-3XFlag-DVL2 which was a gift from Jeff Wrana (Addgene plasmid # 24802) [78 (link)]. Lipofectamine 3000 transfection reagent was purchased from Invitrogen (Carlsbad, CA, USA). BCA protein assay kit, Radioimmunoprecipitation (RIPA) lysis buffer, Nuclear and Cytoplasmic Protein Extraction kit, and protein A+G agarose beads were obtained from Beyotime Biotechnology (Nantong, China). Primary antibodies against FOXM1, E-cadherin, N-cadherin, Vimentin, Snail and P-gp were purchased from Cell Signaling Technology (Danvers, MA, USA). Primary antibodies against DVL2, HA, Flag, GAPDH and Lamin B1 were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The horseradish peroxidase (HRP)-conjugated secondary antibody was purchased from ZSGB-bio (Peking, China).