Si scd1

An SCD1 overexpression plasmid was synthesized by cloning human SCD1 cDNA into plasmid pcDNA3.1 (pcDNA-SCD1), and the empty plasmid pcDNA3.1 served as the vector control. Small interfering RNA (siRNA) targeting SCD1 (si-SCD1, 5′-GCACAUCAACUUCACCACATT-3′) was purchased from Genepharma (Suzhou, China), with a scrambled siRNA (si-Ctrl, 5′-UUCUCCGAACGUGUCACG UTT-3′) used as the negative control. Transfection was performed using Lipofectamine 3000 reagent (Invitrogen, Carlsbad, CA, United States) according to the manufacturer’s protocol.

House dust mite (HDM) extract was purchased from Greer laboratories (Lenoir, NC, USA). Enzyme-linked immunosorbent assay (ELISA) kits for immunoglobulin E (IgE) were purchased from RayBiotech (Norcross, GA, USA). Recombinant human TGF-β1 was purchased from R&D Systems (Minneapolis, MN, USA). The SCD1 inhibitor A939572 and the PI3K inhibitor LY294002 were purchased from MedChemExpress (Shanghai, China). The SREBP1 inhibitor Fatostatin HBr and the mTOR inhibitor Torin1 were purchased from Selleck (Shanghai, China). OA-bovine serum albumin (BSA) was purchased from Sigma‒Aldrich (St. Louis, MO, USA). Small interfering RNA targeting SCD1 (siSCD1) and negative control small interfering RNA (siNC) were obtained from GenePharma (Suzhou, China). The siRNA sequence information is listed in Additional file 1: Table S1. The primers used in this study were synthesized by Synbio Technologies (Suzhou, China). Cytosolic, nuclear and membrane proteins were extracted using a Minute™ Plasma Membrane Protein Isolation and Cell Fractionation Kit (Invent Biotechnologies, Beijing, China) according to the manufacturer’s instructions. The antibodies used are listed in Additional file 1: Table S2.