Liver samples were harvested and fixed in 10% formalin for at least 24 h. Paraffin-embedded tissues were cut into 5-μm-thick sections. Liver sections were rehydrated and processed for antigen retrieval. Endogenous peroxidase was inactivated, and nonspecific signals were blocked using 2.5% horse serum (Vector Laboratories). Then the liver sections were incubated with primary antibodies at 4°C overnight, followed by horseradish peroxidase–conjugated or alkaline phosphatase–conjugated secondary antibodies (Vector Laboratories). All sections were counterstained with hematoxylin. The primary antibodies used were rabbit anti-Ki67, rabbit anti-ST2 (Abcam), and mouse anti-BrdU (Cell Signaling Technology).
Rabbit anti st2
Manufactured by Abcam
Sourced in United Kingdom
Rabbit anti-ST2 is a primary antibody that targets the ST2 protein. ST2 is a member of the interleukin-1 receptor family and is involved in immune response and inflammation. This antibody can be used for the detection and quantification of ST2 in various applications, such as Western blotting and immunohistochemistry.
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Lab products found in correlation
2 protocols using rabbit anti st2
1
Immunohistochemical Analysis of Liver Sections
2
Western Blot Analysis of IL-33 and ST2
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Total protein extracts were prepared as described previously [12] . Equal protein amounts were separated by SDS-PAGE, blotted onto PVDF membranes and analyzed with goat anti-mouse IL-33 (R&D Systems, Minneapolis, Minn., USA; mouse monoclonal antibody, AF3626, 1: 500) , rabbit anti-ST2 (Abcam, Cambridge, UK; rabbit polyclonal antibody, ab25877, 1: 1,000) or rabbit anti-βactin (Cell Signaling, Danvers, Mass., USA; rabbit monoclonal antibody, No. 4970, 1: 4,000) . Appropriate horseradish peroxidaseconjugated secondary antibodies (Dako, Glostrup, Denmark) were used for detection by enhanced chemiluminescence (Amersham Biosciences, Roosendaal, The Netherlands). The band intensity was acquired by a GS710 calibrated imagine densitometer (Bio-Rad).
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