Bone marrow samples were obtained from 19 AML patients and 19 healthy donors from the First Hospital of Wenzhou Medical University. Our study was approved by the ethics committee of the First Hospital of Wenzhou Medical University1.
Quantitative real-time PCR (qRT-PCR) total RNA was extracted using TRIZOL reagent (Life Technologies). reverse transcription was then performed using the HiScript Q RT SuperMix kit (Vazyme, Nanjing, China). qRT-PCR was performed on an Applied Biosystems Quantstudio 6Flex qRT-PCR using a SYBR probe (Applied Biosystems, Foster City, CA, USA). then, qRT-PCR was performed to assess mRNA expression using SYBR Green Master Mix (CWBIO, Jiangsu, China) in an Applied Biosystems QuantStudio 3 Real-Time PCR System (Thermo Fisher Scientific, MA, USA) as previously described.
Quantitative real-time PCR (qRT-PCR) total RNA was extracted using TRIZOL reagent (Life Technologies). reverse transcription was then performed using the HiScript Q RT SuperMix kit (Vazyme, Nanjing, China). qRT-PCR was performed on an Applied Biosystems Quantstudio 6Flex qRT-PCR using a SYBR probe (Applied Biosystems, Foster City, CA, USA). then, qRT-PCR was performed to assess mRNA expression using SYBR Green Master Mix (CWBIO, Jiangsu, China) in an Applied Biosystems QuantStudio 3 Real-Time PCR System (Thermo Fisher Scientific, MA, USA) as previously described.