Antibody against gapdh 60004 1

TRIzol™ RNA extraction reagent, Lipofectamine® 2000 transfection reagent, Lipofectamine® RNAiMAX transfection reagent, Opti-MEM medium, Dulbecco's modified Eagle's medium (DMEM), fetal bovine serum (FBS), and antibody against GFAP (13-0300) were purchased from Thermo Fisher Scientific (Waltham, MA USA). The PrimeScript™ RT reagent kit was purchased from TaKaRa (Kusatsu, Shiga, Japan). SensiFAST™ SYBR was purchased from Bioline (Taunton, MA, USA). Antibodies against α-tubulin (T6199), β-actin (A5316), and HA-tag (SAB4300603) were purchased from Sigma (St. Louis, MO, USA). An antibody against CEBPD (SC-636) was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). An antibody against GAPDH (60004-1) was purchased from Proteintech Group, Inc. (Rosemont, IL, USA). Antibodies against cleaved caspase 3 (#9661) and catalase (#12980) were purchased from Cell Signaling Technology (Danvers, MA, USA). All oligonucleotides were synthesized by PURIGO Biotechnology (Taipei, Taiwan). HA-tagged CAT plasmid was purchased from Sino Biological Inc. (Beijing, China).

Acacetin (purity 98%) was purchased from Tauto Biotech Co., Ltd. (Shanghai, China) and dissolved in DMSO. Primary antibodies against PI3K p85 (4292), EGFR (4267), Met (8198), GSK3β (9315), MMP9 (13,667), p-EGFR (4404 and 2234), Bax (2774), Bcl-2 (17,447), Bcl-xl (2764), Cleaved Caspase-3 (9661), Caspase-3 (9662), Cleaved PARP (5625), PARP (9532), p-STAT3 (9145), STAT3 (9139), p-ERK (4370), Erk1/2 (4695), PCNA (13,110), and Ki-67 (9027), as well as HRP-linked anti-rabbit IgG (7074) and HRP-linked anti-mouse IgG (7076) were purchased from Cell Signaling Technology (Danvers, MA, United States). Antibodies against PTGS2 (D223097), HAVCR1 (D260086), TMPRSS4 (D123375), GJB4 (D260904), KRT17 (D120232), EGR1 (D120585) and AKR1B10 (D221558) were obtained from Sangon Biotech (Shanghai, China). An antibody against GAPDH (60,004-1) was purchased from Proteintech (Wuhan, China). Human EGF (AF-100-15) was purchased from Peprotech (United States).