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2 protocols using acetic acid

1

Soxhlet Extraction and Polyphenol Determination

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Petroleum ether (Carlo Erba, Spain) boiling at 40–70 °C was used for Soxhlet extraction. Chemicals for the determination of polyphenol and antioxidant content were 97% ethanol (Reanal, Hungary), 99% methanol (Reanal, Hungary), anhydrous sodium carbonate (Riedel–de Haen, Germany), Folin–Ciocalteu reagent (Merck, Germany), 2-4-6-tripyridyl-s-triazine (TPTZ) (Sigma-Aldrich, USA), acetic acid (Reanal, Hungary), anhydrous iron chloride (Merck, Germany), 98% Trolox (Sigma-Aldrich, USA), gallic acid (Sigma-Aldrich, USA).
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2

Metronidazole Biomedical Assay Protocol

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Metronidazole of European Pharmacopoeia quality was provided by Aarti Drugs Limited (Mumbai, India). Ferrous sulphate heptahydrate, acetic acid, hydrochloric acid, sodium acetate and perchloric acid of analytical grade was purchased from Reanal (Budapest, Hungary). 2,4,6-tripyridyl-S-triazine (TPTZ), ascorbic acid, 1,1-diphenyl-2-picrylhydrazil (DPPH), hydrogen peroxide, luminol, microperoxidase and thiobarbituric acid (TBA) were obtained from Sigma-Aldrich Ltd. We purchased malondialdehyde (MDA) in the form of diethyl acetate from Merck Ltd.
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